茶菊再生体系及其卡那霉素筛选体系的建立  

Establishment of Regeneration System and Screening System to Kanamycin in Chaju

作　　者：卢龙斗[1] 李爱花[1,2] 彭仁海[2] 高武军[1] 邓传良[1] 周娜[1] 杨绪勤[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]安阳工学院生物与食品工程学院,河南安阳455000

出　　处：《安徽农业科学》2009年第21期9872-9875,共4页Journal of Anhui Agricultural Sciences

基　　金：河南省自然科学基金项目(0611030300)

摘　　要：[目的]建立高效的茶菊再生体系和卡那霉素筛选体系。[方法]以茶菊叶盘和茎段为外植体,以MS为基本培养基,通过添加不同浓度的6-BA和NAA设计不同的培养基,研究茶菊叶盘和茎段的最适分化条件,并进行卡那霉素敏感性试验,研究其对不定芽诱导和生根的影响。[结果]茶菊叶盘和茎段直接分化不定芽的最适培养基分别为MS+6-BA1.0 mg/L+NAA0.3 mg/L和MS+6-BA2.0mg/L+NAA0.1 mg/L,茶菊生根较容易,在5种培养基上生根率均可达100%;茶菊对卡那霉素反应较为敏感,20.0 mg/L卡那霉素即可抑制叶盘的分化,40.0 mg/L的卡那霉素可抑制茎段的分化,30.0 mg/L卡那霉素可抑制小苗的生根。6 cm左右长的生根无菌苗经炼苗和移栽后成活率为100%。[结论]该试验为茶菊的进一步遗传转化提供了基础。[Objective] The study aimed to establish the high efficient regeneration system and screening system to Kanamycin in Chaju. [ Method] With leaf discs and stem segments of Chaju as explants, MS was used as the basic medium and it was supplemented with 6-BA and NAA at different concn, for designing different media in order to study the optimum differentiation conditions of leaf discs and stem segments of Chaju. Kanamycin sensitive tests were carried out to study its effect on adventitious bud induction and rooting. [ Result] The optimum medium for leaf discs and stem segments of Chaju inducing directly adventitious bud differentiation were MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.3 mg/L and MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.1 mg/L, resp. and rooting of Chaju was more easily on 5 kinds of culture medium with the rooting rate of 100% ; Chaju was sensitive to kanamycin, in which, 20.0 mg/L kanamycin could inhibit the differentiation of leaf discs, 40.0 mg/L kanamycin inhibited the differentiation of stem, and 30.0 mg/L Kanamyein inhibited rooting of seedlings. Aseptic seedling about 6 cm in length had survival rate of 100% after hardening and transplanting. [ Conclusion ] The experiment provided a basis for further genetic transformation of Chaju.

关 键 词：茶菊 外植体 组织培养 再生体系 

分 类 号：S603.6[农业科学—园艺学]