白花丹参的组织培养和植株再生  

Tissue Culture and Plant Regeneration of Salvia miltiorrhiza Bge.f.alba

作　　者：梁宏伟[1] 王锋祥[2] 崔秀伟 

机构地区：[1]三峡大学生物技术研究中心湖北省天然产物研究与利用重点实验室,湖北宜昌443002 [2]莱芜职业技术学院生物研究所,山东莱芜271100 [3]山东省莱芜市农业科学技术研究所,山东莱芜271100

出　　处：《安徽农业科学》2009年第21期9876-9876,9904,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]通过植物组织培养技术快速大量繁殖白花丹参优良品系,深度开发利用这一药用植物资源。[方法]以白花丹参的幼嫩叶片和叶柄作为外植体,接种至器官发生分化培养基上诱导丛生芽,然后作壮苗培养,继而生根培养,建立一套快速再生体系。[结果]在MS+6-BA2.0 mg/L+NAA1.0 mg/L和MS+6-BA2.0 mg/L+NAA0.5 mg/L培养基上均能诱导分化大量丛生芽,然后转接至MS+KT1.5 mg/L+NAA0.2 mg/L继代培养基上做继代、壮苗培养,消除玻璃化现象。选取健壮芽苗转接至1/2 MS+IBA0.2 mg/L培养基上生根培养,待试管苗上长出数条2 cm长的健壮根系时即可炼苗移栽,成活率在90%以上。[结论]建立了一套完整的白花丹参组培再生体系。[ Objective ] The research aimed to propagate abundantly excellent line of S. miltiorrhiza Bge. f. alba by plant tissue cuhure, and strengthen the utilization of the officinal plant. [ Method] With the leaf and leafstalk of S. miltiorrhiza Bge. f. alba as explants, the different medium were used to induce clustered buds, strengthen regenerative bud and conduct rooting induction So as to establish a set of rapid propa- gation system. [ Result] The great number of clustered bud were induced at MS medium with supplement 2.0 mg/L 6-BA and 1.0 or 0.5 mg/L NAA. And the buds were transferred to MS medium with supplement 1.5 mg/L KT and 0.2 mg/L NAA in order to strengthen bud and eliminate vitrification of buds. Then rooting induction was conducted at 1/2 MS medium with supplement 0. 2 mg/L IBA. The regenerated plant was exercised and transplant when they outgrew a few 2 cm stocky roots. The survive rate reached over 90%. [ Conclusion] A whole regeneration system of S. miltiorrhiza Bge. f. alba was established.

关 键 词：白花丹参 组织培养 植株再生 

分 类 号：S603.6[农业科学—园艺学]