几种杀菌剂对稻曲病菌的室内毒力测定  

Indoor Toxicity Test of Several Fungicides against Ustilaginoidea Virens

作　　者：李竞生[1] 张敏[1] 陈华保[1] 袁杭[1] 王学贵[1,2] 

机构地区：[1]四川农业大学农学院,四川雅安625014 [2]农药与化学生物学教育部重点实验室,华南农业大学,四川广州510642

出　　处：《安徽农业科学》2009年第21期10042-10044,共3页Journal of Anhui Agricultural Sciences

基　　金：四川山地籼稻丰产高效技术集成与示范(2006BAD02A05);四川省公益性项目(2008FG0017)

摘　　要：[目的]筛选出对稻曲病菌抑菌效果显著的杀菌剂及其最佳混配比例,[方法]从雅安稻田发病严重的稻穗上分离出稻曲病菌株,选择6种杀菌剂以及井冈霉素和烯唑醇不同混配比例,用菌丝干重法对稻曲病菌菌丝的生长进行室内毒力测定,并根据Wadley方法评价混剂的相互作用。[结果]各杀菌单剂对稻曲病菌菌丝的生长均有一定的抑制效果。其中,烯唑醇和井冈霉素对稻曲病菌菌丝生长的抑制效果最好,其EC50值分别为22.07和90.16μg/ml;其次为甲基硫菌灵和三唑酮,其EC50值分别120.89和129.30μg/ml;多菌灵和三环唑抑制效果最差,其EC50值分别为278.04和204.41μg/ml。当井冈霉素和烯唑醇按EC50剂量3∶1和5∶1的比例混配时,其SR分别为1.812 4和1.905 2,表现出较好的增效作用。[结论]该研究为进一步研发防治水稻稻曲病的复配制剂提供科学依据。[Objective] The study aimed to screen the fungicides that bad evident antibacterial effect against Ustilaginoidea virens and their best mixed ratio. [ Method] The U. virens strains were separated from rice panicle that had severe disease in rice field of Yaan, 6 fungicides and validamycin and diniconazole mixed with different ratio were selected to carry out the indoor toxicity test on mycelia growth of U. virens with mycelium dry weight method, and the interaction of mixed fungicides was evaluated according to the method of Wadley. [ Result] Each fungicide had some inhibitory effect on mycelia growth of U. virens. Among which, diniconazole and validamycin had best inhibitory effect on mycelia growth of U. virens, and their EC50 values were 22.07 μg/ml and 90. 16 μg/ml, resp. ; thiophanate-methyl and triadimefon were second and their EC50 values were 120.89 and 129.30μ/ml, resp.; the inhibitory effects of carbendazim and trieyclazole were the worst and their ECso values were 278.04 and 204.41μg/ml, resp. When validamycin and diniconazole were mixed by the ratio of 3：1 and 5：1 according to ECho dosage, their SR values were 1. 812 4 and 1. 905 2, resp. , showing a good synergism. [ Conclusion] The research provided the scientific basis for further development of compound preparations of controlling the rice false smut.

关 键 词：稻曲病菌 杀菌剂 毒力测定 井冈霉素 烯唑醇 多菌灵 

分 类 号：S481.9[农业科学—农药学]