气相色谱法测定搏心通原料药中亚油酸含量  

Determination of Linoleic Acid in Raw Materials of Boxintong by Gas Chromatography

作　　者：王仲[1] 张长弓[1] 付琴琴[1] 王薇[1] 王琼[1] 方伟[1] 

机构地区：[1]华中科技大学同济医学院药学院,武汉430030

出　　处：《医药导报》2009年第7期918-920,共3页Herald of Medicine

摘　　要：目的建立搏心通原料药中亚油酸含量测定的方法。方法采用气相色谱法,色谱柱为:HP-INNOWAX(30m×0.53mm,1μm),程序升温法:120℃保持2min,以10℃·min-1升到180℃,保持2min,以2℃·min-1升到184℃,以1℃·min-1升到190℃;采用甲酯化法对样品进行衍生化处理,并对该衍生方法进行方法学考察。结果亚油酸含量为8.16%,RSD为1.66%。精密度RSD为2.08%,加样回收率为97%～103%。结论该方法操作简便,结果准确可靠。Objective To establish a method for quantitative determination of linoleic acid （LA） in boxintong. Methods The optimal condition was determined by gas chromatography. The column was used as a highly polar （30 m × 0.53 mm,1 μm） fused silica capillary column. The best initial oven temperature was held at 120 ℃ for 2 rain and then increased at 10 ℃ ·min^-1 to 180 ℃ held for2 min, then increased at2 ℃ ·min^-1 to 184 ℃, at last increased at 1 ℃ ·min^-1 to 190 ℃. The samples were defivatized by the method of acid-catalyzed methylations. Results The content of LA was 8.16%, RSD was 1.66% and its precision was under 3%. The recoveries were at ranges of 97% - 103%. Conclusion The proposed method is convenient, accurate and reliable for determination of LA.

关 键 词：搏心通 甲酯化 亚油酸 色谱法 气相 

分 类 号：R286[医药卫生—中药学] R927.2[医药卫生—中医学]