高效液相色谱法测定更昔洛韦葡萄糖注射液的含量  

Contents Determination of Ganciclovir and Glucose Injection by HPLC

作　　者：郭新社[1] 杨珂[1] 李遂一 

机构地区：[1]河南大学药学院,河南开封475004 [2]郑州通泰医药科技有限公司,河南郑州450001

出　　处：《中国药业》2009年第14期30-31,共2页China Pharmaceuticals

摘　　要：目的建立更昔洛韦葡萄糖注射液中更昔洛韦的含量测定方法。方法采用高效液相色谱(HPLC)法测定更昔洛韦的含量。色谱柱为Phenomenex C18柱(250mm×4.6mm,5μm),流动相为水-甲醇(95:5),检测波长为254nm,流速1.0mL/min。结果更昔洛韦进样量在0.5986～1.5962μg范围内与峰面积呈良好的线性关系(r=0.9998),平均回收率为99.86%,RSD=0.27%(n=9)。结论该法简便、准确、重现性好,可用于测定更昔洛韦葡萄糖注射液中更昔洛韦的含量。Objective To establish a method for the determination of ganciclovir in Ganciclovir and Glucose Injection. Methods The content of gancielovir in Ganciclovir and Glucose Injection was determined by HPLC. The separation was performed on Phenomenex C18 column （250 mm ×4.6 mm, 5 μm）, the mobile phase was water-methanol（95 ： 5）, the detection wavelength was 254 nm and the flow rate was 1.0 mL/min. Results There was a good linear relationship between the concentration of ganeiclovir and the areas over the range of 0. 598 6 - 1. 596 2 μg（ r=0. 999 8）. The average recovery rate was 99.86% with RSD =0. 27% （n =9）. Conclusion The method is simple,accurate and reproducible. It can be used for the content determination of ganciclovir in Ganciclovir and Glucose Injection.

关 键 词：高效液相色谱法 更昔洛韦葡萄糖注射液 含量测定 

分 类 号：R927.2[医药卫生—药学] R978.7