磷钒钼蓝光度法测定烟草中的磷  

Determination of phoshorus in tobacco with phosphovanamolybdate blue spectrophotometry

作　　者：刘少民 盛良全 刘济红 戴亚 徐文军 

机构地区：[1]合肥技术经济学院加工系 [2]湖南省烟草公司质量监督检测站

出　　处：《烟草科技》1998年第4期25-27,共3页Tobacco Science & Technology

摘　　要：研究了磷钒钼杂多蓝光度法测定烟草中的磷，较磷钒钼黄光度法的灵敏度高、选择性好、显色液更加稳定，精密度和准确度也获得了满意结果。本法中显色液的最大吸收峰在８１０ｎｍ处，摩尔吸光系数为２．３×１０４Ｌ·ｍｏｌ－１·ｃｍ－１，磷含量在０～３０．０μｇ／２５ｍｌ范围内符合比尔定律，回收率为９８．６％～１０３．２％。A new phosphovanamolybdate blue spectrophotometric method for the determination P in toba cco was studied. The mothod is based on the color reaction of fructose reduced phosphovanamolybdate yellow in sulfuric acid medium. The maximum absorption wavelength of the phosphovanamolybdate blue is 810nm. The value of molar absorption coefficient is 1.94×10 4mol -1 cm -1 .Beer's Law is obeyed in the rang of 0～30 μg/25ml for P. The recovery of phosphorus was 98.6%～103.2%.

关 键 词：磷钒钼杂多酸 分光光度法 烟草 磷 

分 类 号：TS411[农业科学—烟草工业]