软蒺藜中总黄酮的含量测定及其提取工艺的优化  

Study on determination and extraction oftotal flavonoids in seeds of Atriplex centralasiatica

作　　者：赵排风 

机构地区：[1]灵宝市食品药品检验所,河南灵宝472500

出　　处：《河南大学学报（医学版）》2009年第2期115-117,共3页Journal of Henan University:Medical Science

摘　　要：目的:测定软蒺藜中总黄酮的含量并优选提取工艺。方法:采用紫外分光光度法测定软蒺藜中总黄酮的含量,并利用正交实验设计法对影响软蒺藜提取效果的因素进行了研究。结果:测得软蒺藜中总黄酮的含量为0.44%,得到最佳醇提工艺条件是:提取温度80℃,提取时间2 h,乙醇浓度75%。结论:首次从软蒺藜中提取出总黄酮,且该方法操作简便,结果准确,实验结果令人满意。Objective： To determine and select the best process of extraction of the total flavonoid in seeds of Atriplex centralasiatica. Methods： The total flavonoid in the seeds of Atriplex centralasiatica was determined by UV and the orthogonal design was used as guide in the process of extraction of seeds of Atriplex centralasiatica. Results： The contents of total flavonoid in the seeds of Atriplex centralasiatica was 0.41 %, the optimum condition for the extraction of the total flavonoid in seeds of Atriplex centralasiatica was 100ml 75% alcohol, 2 hours for three times under temperature of 80℃. Conclusion： The total flavonoid in seeds of Atriplex centralasiatica is extracted for the first time, and the method is simple and the outcomes of the experiments are satisfactory.

关 键 词：软蒺藜 总黄酮 比色测定法 正交实验 

分 类 号：TQ460.6[化学工程—制药化工]