生存素基因质粒转染对缺血/再灌注神经细胞的保护作用  

Survivin Gene Plasmid Transfection on the Protective Effects to Ischemia-reperfusion of Nerve Cells

作　　者：梅松柳[1] 高俊霞[1] 宋俊兰[2] 

机构地区：[1]牡丹江医学院附属红旗医院急诊科,黑龙江牡丹江157100 [2]牡丹江医学院附属红旗医院检验科,黑龙江牡丹江157100

出　　处：《医学综述》2009年第14期2224-2225,共2页Medical Recapitulate

摘　　要：目的探讨生存素基因Survivin对缺血／再灌注神经细胞凋亡的影响。方法构建真核表达质粒pEGFP—Suvivin，脂质体介导转染细胞，筛选获得稳定表达Suvivin基因的转染细胞，于转染24h制作体外缺血／再灌注模型，于造模后6h对比模型组、空载体组、实验组细胞在Suvivin蛋白、细胞凋亡等方面的变化。观察Suvivin基因对缺血／再灌注神经细胞的抗凋亡作用。结果实验组细胞凋亡率明显低于模型组。结论Suvivin基因转染能有效地保护缺血／再灌注细胞免受凋亡，为其临床应用提供了理论和实验依据。Objective To research the effect of Survivin gene survivin to ischemia-reperfusion of nerve cells apoptosis. Methods To construct the eukarya expression recombinant pEGFP-survivin,, the bangosome mediate transfection cell, to select suvivin gene of transfection cell, at 24 h after transfection to make the model of ischemia-repeffusion, at 6 h after model to contrast the changes of Suvivin proteinum and apoptosis on model group, idler group and test group. To observe the anti-apoptosis effect of suvivin gene to ischemia-reperfusion nerve ceils. Results The apoptosis rate of test group was obviously lower than the model groups. Conclusion Survivin gene transfection can efficiently effect ischemia-reperfusion cells to against apoptosis, can provide theory and experiment base to clinical application.

关 键 词：缺血/再灌注损伤 细胞凋亡 Suvivin基因 

分 类 号：R392.1[医药卫生—免疫学]