牛生长激素基因(bGH)编码区的遗传变异特征  

Genetic Variations of Complete Coding Sequence of Bovine Growth Hormone Gene

作　　者：耿荣庆[1,2] 王兰萍[1] 冀德君[2] 李永红[2] 常春芳[2] 常洪[2] 

机构地区：[1]盐城师范学院生命科学与技术学院,江苏盐城224051 [2]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《云南农业大学学报（自然科学版）》2009年第4期492-496,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：江苏省高校自然科学基础研究项目(08KJB230002);江苏省高校"青蓝工程";国家自然科学基金项目(30571323);盐城师范学院科学研究项目(07YCKL056)

摘　　要：以普通牛、瘤牛、牦牛、大额牛、沼泽型水牛和河流型水牛的代表牛种为对象,探讨不同牛种(或亚种)GH基因编码区序列的遗传变异特征。结果表明,6个群体的GH基因编码区序列全长均为654 bp,共检测到16个SNP位点,外显子1无变异位点,外显子2、外显子3、外显子4和外显子5分别有3个、5个、1个和7个变异位点,表明外显子5是编码区的高变区域,而且SNP位点在不同牛种(或亚种)中的分布存在明显差异。16个核苷酸替代位点中有13个为同义突变,仅有3个非同义突变位点,分别位于第2外显子、第4外显子和第5外显子处,并造成其所编码的氨基酸发生改变。Typical bovine species or subspecies of Bos taurus, Bos indicus, Bos grunniens, Bos frontalis, Bubalus bubalis （Swamp buffalo） and Bubalus bubalis （River buffalo） were used as experi- mental populations to detect genetic variations of complete coding sequence of bGH gene. The results showed that the GH gene was 654 bp in length of all 6 populations and 16 SNP sites were determined from coding region. There was no variation site in the 1 th exon and 3, 5, 1 and 7 variation sites in the 2th exon, 3th exon, 4th exon and 5th exon, respectively. It was indicated that the 5＇h exon was the very high mutation in encoding region. Great difference of the distribution of SNP sites arised between dif- ferent bovine species or subspecies. Of the total 16 substitutions, 13 sites were synonymous and only 3 sites were nonsynonymous. Three nonsynonymous sites were located in the 2th exon, 4th exon and 5th exon, and caused relevant amino acid revised.

关 键 词：牛 GH基因 编码区 单核苷酸多态性 

分 类 号：S823.2[农业科学—畜牧学]