芽孢杆菌两菌株对香蕉炭疽病菌的抑制作用及其机制  

Inhibitory Effect and Mechanism of Two Bacillus sp. Strains on Colletotrichum musae

作　　者：许曼琳[1] 段永平[1,2] 吴祖建[1] 谢荔岩[1] 谢联辉[1] 林奇英[1] 

机构地区：[1]福建农林大学植物病毒研究所,生物农药与化学生物学教育部重点实验室,福建福州350002 [2]美国佛罗里达大学植物病理系,美国盖恩斯维尔市32611

出　　处：《云南农业大学学报（自然科学版）》2009年第4期522-527,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：闽计投资项目[(2002)153号];福建省科技厅重点实验室资助项目(2007L2002)

摘　　要：分离了两个芽孢杆菌（Bacillus sp．）菌株25-1和25-17，均对香蕉炭疽病菌（Colletotrichum musae Berk．et Curt．）有较强的拮抗作用。平板对峙培养出现明显的抑菌环；以凹玻片法，用两菌株培养滤液处理病原菌分生孢子，其孢子萌发率降低53％-69％；将细菌培养滤液和孢子悬浮液涂布于PDA平板上，则两者均能抑制香蕉炭疽病菌分生孢子的形成。普通光学显微镜和扫描电子显微镜观察显示，两菌株均可抑制香蕉炭疽病菌菌丝生长，造成菌丝分支增多，顶端和中央膨大，随着时间的延长畸变结构增加，菌丝成念珠状或菌丝分支显著增多，且顶端细胞变形膨大聚集成簇，菌丝壁穿洞，内含物质泄漏，终使细胞崩解和消融，变成球状的空泡。Two strains of Bacillus sp. （25 -1 and 25 -17） had strong antagonistic activities against Colletotrichum musae on agar plates with an apparent inhibitory zone. The rate of conidium germination reduced by 53% -69% when the culture filtrate from these two isolates mixed with conidia solution in glass slide. The conidia formation was inhibited in the PDA agar plate with the culture filtrate and spores. Using optical microscope and scanning electronic microscope, the hyphae were revealed of growth suppression, increasing branches and swelling in the middle and/or the top treated by the two strains. The longer time the treatment, the more severe the hyphae aberration were, some of them were altered beadlike, some offset formed cluster-like structure. The hyphal wall necrosis with the cytoplasm leakage, and some of them finally became global vacuole.

关 键 词：芽孢杆菌 香蕉炭疽病菌 抑制作用 机制 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]