微环境调控对半夏无糖组培苗光合自养的影响  

Effect of Microenvironment Control on the Photoautrophic Growth of Plantlet in Sugar-free Tissue Culture of Pinellia ternate(Thunb.)Breit.

作　　者：占艳[1] 王荔[1] 陈疏影[1] 杨艳琼[1] 和世平[1] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2009年第4期539-544,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省自然科学基金资助项目(2005C0034M)

摘　　要：以半夏叶片通过组织培养产生的不定芽为材料，应用植物无糖组培快繁技术，研究探讨了不同光照强度、不同光照时间对半夏无糖组培苗光合生理的影响。结果表明：当光照强度在37．5—112．5μmoL／（m2·s）范围内，半夏无糖组培苗净光合速率、光合色素含量与光照强度呈正相关；随着光照强度的增加，半夏无糖组培苗的蒸腾速率逐渐下降、气孔导度也逐渐减小；随着光照强度增强，半夏无糖组培苗的生长速率明显加快。无糖组培苗苗高、叶面积、主根数及须根数与有糖组培苗相比均显著增加，且无糖组培苗长出大量须根，而有糖组培苗无须根。In this research, adventitious bud of Pinellia ternate （Thunb.） Breit. was used as material to study the effect of different light intensities and photoperiods on the photophysiology of leaf and root of plantlet in sugar-free tissue culture of P. ternate. The result showed that the photosynthetic rate and chlorophyllous contents of plantlet in sugar-free tissue culture of P. ternate plantlet increased obviously with the increase of light intensity [37.5 ～ 112.5μmol/ （m2·s）] ; the transpiration rate and stomatal condance of plantlet in sugar-free tissue culture of P. ternate was lower than that in sugar tissue culture （CK） ; the rate of growth of the plantlet of P. ternate in sugar-free tissue culture increased significantly. The area of leaf and the numbers of root and fibrous increased obviously. While CK had no fibrous, the plantlet in sugar tissue culture had weaker root system.

关 键 词：半夏 无糖组织培养 光照强度 光周期 光合生理 

分 类 号：S567.239.035.3[农业科学—中草药栽培]