番茄斑萎病毒属病毒检测技术研究进展  

Advances on Detection Techniques for Tospovirus

作　　者：郑元仙[1] 刘雅婷[1] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2009年第4期607-613,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省自然基金项目(2005C0036M;2007C0036M);云南省教育厅自然基金项目(5Y0171B)

摘　　要：番茄斑萎病毒属病毒属于布尼亚病毒科,是一类世界性分布、寄主范围很广的植物病毒,可引起许多重要的园艺植物和农作物的严重病害,造成严重的经济损失。而高效的病毒鉴定或检测技术是防治病毒病害的关键基础之一。因此,本文就近年来番茄斑萎病毒属病毒鉴定与检测技术的研究进展作一综述,介绍生物学技术、电镜技术、血清学技术以及分子生物学技术在番茄斑萎病毒属病毒检测方法上的特点、发展应用方向等。Genus Tospovirus belonging to the family Bunyaviridae is an important plant virus genus. Tospovirus has wide ranges of natural hosts and can infect agricultural important crops and garden plants and cause great economic loss worldwide. The highly effective viral appraisal or the examination tech- nology is one of essential foundations for preventing and controlling plant viral disease. Thus, the advances on the techniques and methods to detect tospoviruses were reviewed in this paper. The characters of the methods commonly used to tospoviruses diagnosis, such as biological techniques, electron microscope, immunological methods and molecular biological techniques and so on, and their direction of development and application were introduced.

关 键 词：番茄斑萎病毒属 番茄斑萎病毒 生物学检测 电镜技术 血清学技术 聚合酶链式反应 

分 类 号：S432.41[农业科学—植物病理学]