脂多糖刺激对牛单核细胞由来的巨噬细胞Toll样受体表达的影响  

Toll-like receptor expression pattern in bovine monocytes-derived macrophages and the influence by Lipopolysaccharid stimulation

作　　者：郭熠洁[1] 田中沙智[2] 赵国琦[1] 

机构地区：[1]扬州大学动物科学与技术学院反刍动物营养研究室,扬州225009 [2]日本东北大学机能形态实验室,981-8555

出　　处：《中国免疫学杂志》2009年第7期638-640,646,共4页Chinese Journal of Immunology

基　　金：日本文部省反刍家畜研究项目;国家自然科学基金(30871800);国家科技支撑项目(2006BAD04A03-07)

摘　　要：目的:研究牛末梢血中单核细胞由来的巨噬细胞在受到LPS刺激后细胞表面Toll样受体表达的变化。方法:试验采取3头日本黑牛外周血,进行分离得到外周血单核细胞,并用Repcell进行巨噬细胞的分离培养,7天后用脂多糖(lipopolysaccharide,LPS)刺激细胞24小时后,通过RT-PCR测定巨噬细胞表面Toll样受体mRNA的表达以及细胞因子mRNA的表达。结果:在LPS刺激24小时后,IL-6、TNF-α和IL-1βmRNA的表达量显著升高(P<0.05),与此同时IL-8和IL-12p40 mRNA的表达量相对提高;TLR1和TLR10的转录物显著下降(P<0.05),然而TLR2、4和6保持稳定。结论:巨噬细胞在受到病原刺激后通过提高IL-6、TNF-α和IL-1βmRNA的表达量,从而提高了天然免疫的作用。该研究为探讨巨噬细胞在无然免疫中的作用奠定了基础。Objective： To examine Toll-like receptor expression pattern in monocytes-derived macrophages by Lipopolysaccharid （LPS） stimulation. Methods： Jugular venous blood was collected from three Japanese calves, and then the peripheral blood mononuclear cells （PBMC）, were isolated,and cultured for 7 days to collect monocytes-derived macrophages in Repcell and the cells were stimulated with LPS for 24 h to analyze the mRNA expression pattern of TLR in monocytes-derived macrophages. Results： The mRNA expressions of IL-6, TNF-α and IL- 1β were significantly increased after LPS stimulation（ P 〈 0.05）, while IL-8 and IL- 12 p40 was relatively augmented. The TLR1 and 10 transcripts were significantly decreased after LPS stimulation（ P 〈 0.05）. There was no difference in the mRNA expression of TIB2,4 and 6 between non-treatment and LPS stimulation. Conclusion： Monocytes-derived macrophages enhance IL-6, TNF-α and IL-β mRNA expression to increase the innate immtme activation by product cytokines. The important function of the cell could contribute to better disease treatment.

关 键 词：单核细胞由来的巨噬细胞 牛 TOLL样受体 天然免疫 

分 类 号：R392.9[医药卫生—免疫学]