杂交水稻苗期耐冷性综合评价及其鉴定指标的筛选  

Comprehensive Evaluation and Screening Identification Indexes of Cold Tolerance at Seedling Stage in Rice

作　　者：傅泰露[1] 马均[1] 李敏[1] 童平[1] 吴合洲[1] 李树杏[1] 陈宇[1] 朱萍[1] 

机构地区：[1]四川农业大学水稻研究所,四川温江611130

出　　处：《西南农业学报》2009年第3期608-614,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家科技支撑计划重大项目(2006BAD02A05);四川省育种攻关项目(2006yzgg-28)

摘　　要：利用不同类型的21个水稻品种,采用人工低温胁迫和自然低温胁迫的方法,测定了叶龄、苗高、叶面积等8项与耐冷性相关的形态指标;选取了5个不同类型的水稻品种,测定了叶绿素含量、可溶性糖含量、可溶性蛋白质含量、脯氨酸含量、超氧化物歧化酶(SOD)、丙二醛(MDA)等7项与耐冷性相关的生理生化指标,利用相关性显著的指标进行了主成分分析和逐步回归分析。结果表明,水稻苗期叶龄和叶片丙二醛含量与水稻耐冷性有显著关系,可作为水稻苗期的耐冷性鉴定指标。利用所建立的最优回归方程对供试品种进行了预测,结果与他们的存活率表现基本一致,表明用这两个指标在苗期对水稻耐冷性进行鉴定是可行的,用该方法对水稻耐冷性进行综合评价也是可行的。In this paper, eight morphological indexes correlating with cold tolerance including leaf age , plant height, root number, leaf area were monitored under low-temperature stress at seedling stage for 21 rice cultivars and seven physiological and biochemistry indexes correlating with cold tolerance including Chl content, soluble sugar content, soluble protein content, proline content, superoxide dismutase（SOD） activities, malondialdehyde content were monitored under low-temperature stress for 5 rice cultivars. The indexes that were significandy correlated with their cold tolerance were screened by the main content analysis and the step regression analysis. The results showed that at seedling stage leaf age and MDA content were significandy correlated with their cold tolerance and could be selected to identify the cold tolerance of rice. The cold tolerance of 5 rice cultivars was evaluated by stepwise regression equation and the results were basically consistent with the results in their livability rate,which indicated that it was feasibleto predict the cold tolerance in rice at seedling stage using the two indexes screened out in this study and the way that this paper used was feasible to comprehensive evaluate the cold tolerance in rice.

关 键 词：水稻 耐冷性 苗期 综合评价 鉴定指标 

分 类 号：S511.034[农业科学—作物学]