钙离子及pH值对柚木组培苗生长和矿质营养吸收的影响  

Effects of Calcium Ions and pH Values on the Growth and Nutrient Uptake Of Teak (Tectona grandis) Tube Seedlings

作　　者：周再知[1] 徐大平[1] 梁坤南[1] 黄隆斌[2] 裘珍飞[1] 

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520 [2]昆士兰大学矿区恢复研究中心

出　　处：《中南林业科技大学学报》2009年第3期1-5,共5页Journal of Central South University of Forestry & Technology

基　　金：国家"十一五"科技支撑专题"柚木珍贵用材林高效培育技术"(2006BAD24B0901)部分内容

摘　　要：利用柚木继代组培试管苗，调整组织培养基本培养基（Ms）配方中的Ca^2＋浓度和pH值，采用随机区组试验设计，开展了5个Ca^2＋浓度（0，3，6，9，12mmol·L-1）和4个pH值水平（pH值4．0，pH值5．0，pH值6．0，pH值7．0）双因素试验，目的是探讨不同Ca2＋浓度与pH值对柚木组培苗生长和矿质营养吸收的影响．结果表明：幼苗的高生长及生物量积累与Ca^2＋浓度呈正相关，最适Ca^2＋浓度为9mmol·L-1；pH值对苗高生长及愈伤组织生物量积累有显著影响，适宜pH值为6．0；茎叶的钙含量随Ca^2＋浓度的增加而增加，与培养基的pH值高低无必然关系；在pH值6．0培养条件下，低至中等Ca^2＋浓度（3～6mmol·L^-1）有利于幼苗对N，P，Mg，Mn，zn，B，Cu营养元素的吸收，较高的Ca^2＋浓度有利于提高钾离子吸收的选择性及提高N吸收量，幼苗对铁离子的吸收随着Ca^2＋浓度的增加而下降．A two-factor experiment was undertaken by employing randomized block design to test the growth and nutrient-uptake responses of teak （Tectona grandis） seedlings in vitro to 0, 3, 6, 9 and 12 mmol· L-1 of calcium concentrations and pH treatments of subculture medium. Resuhs indicate that the height increments and dry weights of leaf and stem are positively related to the Ca^2＋ concentration, the optimal of which is 9 mmol· L-1; that the height growth is significantly affected by pH values, the optimal of which is 6 ; that the total Ca content in leaf and stem is enhanced with the increase of Ca^2＋eoncentration in subculture medium, having no necessary relation with low pH values; that 3-6 mmol · L-lof Ca2＋eoncentration is favorable for teak tube seedlings to uptake N, P, Mg, Mn, Zn, B and Cu elements and a high Ca^2＋concentration, 9 mmol ·L-1 , is beneficial for K-ion uptake; and that Fe-uptake is markedly restrained with the increase of Ca^2＋ concentration.

关 键 词：柚木 组培苗 钙 PH值 生长 矿质营养吸收 

分 类 号：Q945.3[生物学—植物学]