美洲大蠊提取物对3株人体生殖系统肿瘤细胞的细胞毒性研究  

Study on systena genitale tumor cell cytotoxicity of extracts from Periplaneta americana

作　　者：何正春[1] 王晓雨[2] 杨雷香[2] 赵昱[2] 刘光明[1] 

机构地区：[1]大理学院药学院,云南大理671000 [2]浙江大学药学院,浙江杭州310058

出　　处：《西北药学杂志》2009年第4期271-272,共2页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金资助(No:30560181)

摘　　要：目的通过对美洲大蠊提取物的肿瘤细胞毒性测试,探讨美洲大蠊提取物的抗肿瘤作用。方法美洲大蠊醇提物,通过聚酰胺柱层析分离划段得不同部位,采用MTT法对所得部位进行肿瘤细胞毒性测试。结果有多个部位样品的IC50值小于10mg·mL-1。结论通过样品与对照品细胞毒活性结果比较,表明美洲大蠊提取物中存在具有肿瘤细胞生长抑制作用的物质,值得对其进行进一步的研究。Objective To study the anti-tumor activity of extracts from Periplaneta americana by cytotoxicity assay on systena genitale tumor cell （HO8910, Hela, PC3）. Methods The samples are prepared with polyamide column chromatography separation of extracts from Periplaneta americana and the cytotoxicity of samples assessed with MTT assaying. Results There are several samples have strong cytotoxicity and some of them have IC50 lower than 10 mg · L^-1. Conclusion There are anti-tumor activity compounds in extracts of Periplaneta americana by comparison the cell cytotoxicity results.

关 键 词：美洲大蠊 提取物 MTT 细胞毒 

分 类 号：R965[医药卫生—药理学]