番木瓜抗病突变抗PRSV基因的RAPD标记分析  

RAPD analysis on a gene of resistance to PRSV in Papaya

作　　者：黄建昌[1] 肖艳[1] 夏有杰 

机构地区：[1]仲恺农业工程学院农业与园林学院,广东广州510225 [2]湖北省通山县农业局,湖北通山437600

出　　处：《仲恺农业工程学院学报》2009年第2期1-4,共4页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广东省科技计划农业攻关(2002A208010201)资助项目

摘　　要：利用随机扩增多态性DNA(Randomly amplified polymorphic DNA,RAPD)分子标记和集团分离分析法(Bulked segregation analysis,BSA)分析技术,对番木瓜(Carica papaya L.)F_2代分离群体和抗番木瓜环斑型花叶病毒(Papaya ringspot virus,PRSV)突变体LK-1的抗PRSV基因进行连锁分析,从250个随机引物中筛选出了与抗PRSV基因相连锁的3个RAPD标记,连锁分析表明,S1366、S7058和S7125与抗PRSV基因(rys)的遗传距离分别为1.9 cM、2.4 cM和12.2 cM,其中S1366和S7058两个标记在抗PRSV基因的一侧,S7125在另一侧。The segregated population of hybrids was developed by crossing and was analysed by using randomly amplified polymorphic DNA and Bulked segregation analysis methods. 250 random primers were used to detect molecular markers linked to resistant gene in the natural mutant Lk-1. The results showed that polymorphic bands amplified with the three primers were S1366, S7058 and S7125. Of the bands, S7058-700 bp and S7125-600 bp were found in the susceptible pool and susceptible plants of F2 population （ 127 plants）, while not found in the resistant pool and resistant plants of F2 population. However, S1366-900 bp was not found in the susceptible pool and susceptible plants of F2 population, but found in the resistant pool and resistant plants of F2 population. The result showed that the 3 markers were closely linked to the rys gene and that the genetic distance between S7058 700 , S7125 6000, S136 900 and rys gene was 1.9 cM, 2. 4 cM and 12. 2 cM respectively. S7058 700 and S136 900 were at the flank of the rys gene locus and S7125 600 was at the other flank of the rys gene locus.

关 键 词：番木瓜(Carica PAPAYA L.) PRSV RAPD BSA 连锁分析 

分 类 号：S669.7[农业科学—果树学]