油茶优良无性系叶片愈伤组织诱导研究  

Study on Leaf Callus Induction of Camellia oleifera Superior Clone

作　　者：王瑞[1] 陈永忠[1] 王湘南[1] 彭邵锋[1] 杨小胡[1] 王玉娟[1] 杨杨[1] 

机构地区：[1]湖南省林业科学院,湖南长沙410004

出　　处：《经济林研究》2009年第2期35-39,共5页Non-wood Forest Research

基　　金：国家科技部科技支撑项目"油茶高产优质培育技术研究"(2006BAD18B02-04);国家科技部重点推广项目"湘林系列油茶新品系繁育及配套丰产技术示范与推广"

摘　　要：以油茶优良无性系湘林1号和湘林4号为材料,采用正交设计试验对其叶片进行离体培养并诱导形成愈伤组织。试验结果表明:激素种类与浓度组合对愈伤组织诱导影响很大,且两个品种表现不一致,湘林1号以IBA0.01mg·L-1+KT0.5mg·L-1+2,4-D0.5mg·L-1+NAA2.0mg·L-1最佳,湘林4号以IBA0.1mg·L-1+6-BA0.5mg·L-1+KT1.0mg·L-1+NAA2.0mg·L-1最佳,诱导率均达100%。低温预处理5～10d有助于愈伤组织的诱导,不同光照条件以及叶片不同部位均对愈伤组织的诱导有影响。Fresh leaves of explants from ＇xianglin 1＇ and ＇xianglin 4＇ of Camellia oleifera clones were cultured and callus were induced. The results showed that different types and consistency of hormone had a great influence on callus induction, and there was great difference between ＇xianglin 1＇ and ＇xianglin 4＇ , for ＇xianglinl＇ IBA 0.01mg·L^-1＋ KT 0.5 mg·L^-1 ＋ 2,4-D 0.5 mg·L^-1＋ NAA 2.0 mg·L^-1 was the best, while ＇xianglin 4＇ was IBA 0.1mg·L^-1 ＋ 6-BA 0.5 mg·L^-1＋ KT 1.0 mg·L^-1＋ NAA 2.0mg·L^-1, their induction rate reached 100%. Pretreatment with low temperature from five to ten days contributed to callus induction, different light conditions and different parts of leaf had influence on callus induction.

关 键 词：油茶 优良无性系 叶片 愈伤组织诱导 

分 类 号：S794.4[农业科学—林木遗传育种]