油茶种子辐射对幼苗两种同工酶活性的影响  

Effects of ^(60)Co-γ Radiation on Isozyme Activity of Camellia Oleifera Seedling

作　　者：黄永芳[1] 袁中桂[1] 钟伟良[2] 张晖[1] 董斌[1] 

机构地区：[1]华南农业大学经济林研究中心,广东广州510642 [2]华南农业大学生命科学学院,广东广州510642

出　　处：《经济林研究》2009年第2期85-87,共3页Non-wood Forest Research

基　　金：广东省林业科技创新专项资金项目(2008kJX007-01);广东省科技攻关项目(2007B020711003;2008A020100009)

摘　　要：用60Co-γ射线辐照3个无性系及优树的种子,分别以1000rad、2000rad、3000rad和4000rad4个剂量辐照及对照处理,测定1年生油茶幼苗叶片的SOD和POD酶活性。结果表明:在1000rad辐照下,软枝2和3号油茶的幼苗叶片SOD酶活性升高,3000rad辐照的酶活性下降,软枝11号在2000rad时最高,粤15号3000rad时最高;软枝2号和3号在1000rad辐照下,POD酶活性最高,而软枝11号和粤15号POD酶活性则相反,在3000rad辐照下POD酶活性最高。Seeds of Camellia Oleifera from four families were treated by ^60Co-γ ray with a gradient of doses （1 000, 2 000, 3 000, 4 000 tad）. Seeds were sowed after radiation, and superoxide dismutase and peroxidase of leaves were measured after one year growth of the seedlings. As the results showed： SOD activity in seedling leaves of ＇Ruanzhi 2＇ and ＇Ruanzhi 3＇ increased under 1 000 rad, decreased under 3 000 rad. ＇Ruanzhi 11＇ was the highest under 2 000 rad, ＇Yue 15＇ was the highest under 3 000 rad＇ POD activity in seedling leaves of ＇Ruanzhi 2＇ and ＇Ruanzhi 3＇ was the highest under 1 000 rad, ＇Ruanzhi 11＇ and ＇Yue 15＇ were the lowest under 3 000 rad.

关 键 词：油茶 60Co-γ射线辐照 过氧化物酶 超氧化物岐化酶 

分 类 号：S794.4[农业科学—林木遗传育种]