MAPK转导通路在白念珠菌菌体抗氧化应激中的作用  

The role of MAPK transduction pathway in the oxidative stress response in Candida albicans

作　　者：陈裕充[1,2] 温海[1] 高平挥[3] 章强强[2] 顾菊林[1] 陈江汉[1] 郑志忠[2] 

机构地区：[1]第二军医大学附属长征医院皮肤科,上海200003 [2]复旦大学附属华山医院皮肤科,上海200040 [3]第二军医大学药学院药理教研室,上海200433

出　　处：《中国真菌学杂志》2009年第3期137-139,170,共4页Chinese Journal of Mycology

基　　金：国家博士后基金(20060400577)

摘　　要：目的探讨MAPK通路在念珠菌抗氧化应激中的作用。方法采用不同浓度过氧化氢刺激白念珠菌,通过流式细胞仪检测念珠菌的凋亡率,并计算其增殖指数;通过实时荧光定量PCR检测MAPK通路中8种基因的表达水平。结果随着过氧化氢的刺激浓度增高,白念珠菌的凋亡率逐渐升高,而其增殖指数下降。在不同的过氧化氢浓度刺激下,MAPK通路中各基因表达水平基本一致,即在较低的过氧化氢浓度刺激下,各基因表达水平均有一定的上升,而随着浓度增高,在高浓度的过氧化氢刺激下,各基因表达水平趋于稳定。结论在低浓度的过氧化氢刺激下,白念珠菌的凋亡率虽有所上升,但其相应的增殖指数也有所上升,即生长加快。这可能与其MAPK通路中各基因表达增强有一定的关系。Objective To explore the role of MAPK transduction pathway in the antioxidative stress of Candida albicans. Methods The apoptosis rate was detected by the flow cytometry, and the proliferation index was calculated. Using RT-PCR, the transcriptional levels of MAPK genes were examined. Results The apoptosis rate of Candida albicans rose up while its proliferation index dropped down with the concentration of H2 02 increasing. Transcriptional levels of genes in MAPK passway went up at stimulus of low concentration of hydrogen dioxide, while became stable with the concentration increasing. Conclusions At low concentration of hydrogen dioxide stimulus, increase of apoptosis rate and proliferation index was consistent with the transcriptional levels of MAPK genes in Candida albicans .

关 键 词：念珠菌 应激反应 转导通路 基因 

分 类 号：R379.4[医药卫生—病原生物学]