人巨细胞病毒UL143基因在临床低传代分离株中的多态性研究  

Sequence variability of human cytomegalovirus UL143 open reading frame gene in low-passage clinical isolates

作　　者：何蓉[1] 阮强[1] 齐莹[1] 马艳萍[1] 吉耀华[1] 

机构地区：[1]中国医科大学附属盛京医院病毒室,沈阳110004

出　　处：《中华微生物学和免疫学杂志》2009年第6期491-494,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目（30700916）

摘　　要：目的探讨人巨细胞病毒（human cytomegalovims，HCMV）UL143序列在临床患儿低传代分离株中的多态性及其与临床疾病的关系。方法对19株HCMV临床低传代分离株进行HCMV-UL143PCR扩增分析及全序列测定分析。结果19株HCMV感染患儿临床分离株均因碱基插入造成移码突变，开放阅读框架（ORF）比Toledo株短。根据序列变异情况可将19个序列分为2组，第1组16个序列新增一个MYRISTYL位点；缺失2个PKC磷酸化位点。未发现黄疸、小头畸形、先天性巨结肠等不同疾病类型的序列之间的差异。结论HCMV-UL143较多存在于临床低传代分离株中，序列呈现一定多态性。Objective -To explore the relationship between UL143 sequence variability and clinical disease. Methods ULld3 from samples obtained from suspected congenitally human cytomegalovirus （HCMV） infected symptomatic infants were PCR amplified and sequenced. Results There were not too much sequence variability of UL143 compared with Toledo. But no one was completely identical to Toledo, and all ULld30RFs were shorter than Toledo for frame-shift. Conclusion HCMV-UL143 existed in most of low passage isolates and sequences were variable. No obvious linkage was observed between UL143 polymorphisms and outcome of suspected congenital HCMV infection.

关 键 词：人巨细胞病毒 UL143基因 多态性 

分 类 号：R72[医药卫生—儿科]