阿仑膦酸钠对髋关节假体周围界膜组织分泌肿瘤坏死因子α的影响  

Effects of Alendronate sodium on tumor necrosis factor alpha secreted from periprosthetic interface membranes of hip joints

作　　者：李前龙[1] 陈超[2] 

机构地区：[1]广东医学院外科教研室,广东省东莞市523808 [2]南方医科大学中医药学院骨伤推拿教研室,广东省广州市510515

出　　处：《中国组织工程研究与临床康复》2009年第26期5031-5034,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:目前肿瘤坏死因子α的研究有望成为防治假体周围骨丢失的新的切入点,但阿仑膦酸钠对界膜分泌肿瘤坏死因子α的影响及作用机制尚不清楚。目的:观察阿仑膦酸钠对髋关节假体周围界膜组织分泌肿瘤坏死因子α的影响。设计、时间及地点:随机分组设计,对比观察,实验于2006-02/03在广州中医药大学附属骨科医院骨科实验室完成。材料和对象:界膜组织(15g)取自广州中医药大学第一附属医院(患者知情并同意);雄性新西兰白兔6只用于制备阿仑膦酸钠含药血清,阿仑膦酸钠为石家庄制药厂产品。方法:无菌条件下从人工髋关节假体周围股骨分离界膜组织(15g),并将其放入RPMI培养液中培养,将界膜组织剪成约250mg的碎块,计30块,随机分为3组:空白对照组、低浓度阿仑膦酸钠组(100g/L)、高浓度阿仑膦酸钠组(200g/L),每组10个样本。低浓度阿仑膦酸钠组和高浓度阿仑膦酸钠组分别加入1.8mL和1.6mL,空白对照组加入1.8mL。然后在低浓度阿仑膦酸钠组、高浓度阿仑膦酸钠组的培养孔中分别加入0.2mL和0.4mL的阿仑膦酸钠含药血清,空白对照组加入0.2mL空白血清。添加血清后在37℃、含体积分数为5%CO2的饱和湿度条件下培养72h。主要观察指标:酶联法检测各组界膜组织中肿瘤坏死因子α的表达。结果:低浓度阿仑膦酸钠组和高浓度阿仑膦酸钠组界膜组织分泌肿瘤坏死因子α的量分别为(3.93±0.03),(3.92±0.04)pg/g,与空白对照组(4.04±0.13)pg/g相比,差异有显著性意义(P<0.01)。结论:阿仑膦酸钠能显著抑制髋关节假体周围界膜组织分泌肿瘤坏死因子α。BACKGROUND： Studies regarding tumor necrosis factor a （TNF-α） is a newly entrance point in preventing and curing the difficult clinical problems of periprosthetic osteolysis. However, studies about the effect and mechanism of alendronate sodium on TNF-α are few. OBJECTIVE： To explore the effect of alendronate sodium on TNF-αsecretion of periprosthetic interface membranes of hip joints. DESIGN, TIME AND SETTING： A single sampte contrast observation was performed at the Laboratory of Orthopedics in the Affiliated Orthopedic Hospital of Guangzhou University of Chinese Medicine. MATERIALS AND PARTICIPANTS： Periprosthetic interface membranes （15 g） were obtained from the First Affiliated Hospital of Guangzhou University of Chinese Medicine; 6 male New Zealand white rabbits were prepared for serum containing alendronate sodium. The alendronate sodium was manufactured by Shijiazhuang Pharmaceutical Factory. METHODS： Periprosthetic interface membranes （15 g） dissected aseptically from femurs were cultured in RPMI culture medium, periprosthetic interface membranes were sectioned into 30 fragments with weighing about 250 mg per fragment. The 30 fragments were randomly divided into control group, low concentration group （100 g/L） and high concentration group （200 g/L）, with 10 samples in each group. Totally 1.8 mL, 1.6 mL and 1.8 mL culture medium were added into low concentration, high concentration and control groups respectively. Then 0.2 mL, 0.4 mL serum containing Alendronate was added respectively in low concentration, high concentration groups, 0.2 mL control serum was added in the control group. After addition of serum, the tissue suspension was incubated at 37 ℃in saturation humidity with 5% CO2 for 72 hours. MAIN OUTCOME MEASURES： Expression of TNF-α in periprosthetic interface membranes of all groups were measured using ELISA methods. RESULTS： TNF-α secretion of periprosthetic interface membranes in low concentration, high concentration groups were （3.93±0.03）, （3.9

关 键 词：阿仑膦酸钠 界膜:肿瘤坏死因子α 

分 类 号：R318[医药卫生—生物医学工程]