用于再灌注损伤研究的两种肝移植模型的筛选  

Comparison study on two liver transplantation models for reperfusion injury

作　　者：冯敏[1] 陶立德[1] 张培建[1] 刘新颜[1] 金成[1] 郎洁[1] 

机构地区：[1]扬州大学第二临床医学院普通外科,江苏扬州225001

出　　处：《中国现代普通外科进展》2009年第6期469-472,共4页Chinese Journal of Current Advances in General Surgery

基　　金：江苏省卫生厅医学科研招标立项课题(H200770);扬州市科技攻关项目(YZ2008042)

摘　　要：目的:筛选一种用于研究肝移植过程中缺血再灌注损伤的大鼠肝移植模型。方法:将SD大鼠随机分为假手术组(S组)、原位肝移植组(OLT组)与自体肝移植组(AT组)。比较各组大鼠手术时间、存活率及术后24h血清中总胆红素(TB)、谷丙转氨酶(ALT)、谷草转氨酶(AST)及血清肿瘤坏死因子-α(TNF-α)含量,观察肝组织形态学改变。结果:与S组相比OLT组、AT组术后血清TB、ALT、AST升高明显,AT组较OLT组术后存活率明显提高,血清TB、ALT、AST及TNF-α含量明显降低,肝形态异常变化减轻(P<0.05)。结论:自体肝移植模型手术简单、成功率高、可比性强,且排除了免疫损伤的影响,更好地反映了肝脏缺血再灌注损伤的病理生理过程,比较适宜作为肝移植缺血再灌注损伤研究的动物模型。Objective： To select a mope suitable liver transplantation model for studying ischemic reperfusion injury. Methods： SD rats were randomly divided into three groups： sham operation group （S）, orthotopic liver transplantation group （OLT） and analogic liver transplantation group （AT）. The requirement for micro-surgical skills, survival rate and deterioration of histomorphology after operation among each group were observed. At the same time. the levels of total bilirubin （TB）, alanine aminotransferase （ALT）, glutamic oxalacetic transaminase （AST） and tumor necrosis factor- α （TNF- α） in the serum at 24 hours after operation were recorded. Results： The levels of TB, ALT, AST and TNF- α in AT group and OLT group were higher than those in S group. In AT group the pathological changes were milder and the levels of TB, ALT,AST,TNF-α were lower in AT group （P〈0.05）comparing with OLT, and AT group required micro -surgical skills and had higher survival rate （P〈0.05 ）. Conclusions： AT simplifies the really reflects the pathophysiologic course of reperfusion injury , therefore it can be chosen as an ideal model for studying ischemic reperfusion injury in liver transplantation .

关 键 词：肝移植 再灌注损伤 大鼠 

分 类 号：R617[医药卫生—外科学]