Microsporogenesis and meiotic behavior in nine species of the genus Pinus  

作　　者：Hui-Sheng DENG Da-Ming ZHANG De-Yuan HONG Cheng-Xin FU 

机构地区：[1]College of Life Science, Zhejiang University, Hangzhou 310029, China [2]Department of Chemistry and Life Science, Liuzhou Teachers College, Liuzhou 545003, China [3]State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

出　　处：《Journal of Systematics and Evolution》2009年第4期287-290,共4页植物分类学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(GrantNo.30121003)

摘　　要：The meiotic behavior of 10 taxa （nine species and one variety） of the genus Pinus was investigated using pollen mother cells （PMCs） to reveal the differentiation among karyotypes. Chromosome spreads were prepared by conventional squashing. The meiotic index and the average configuration were higher, whereas the frequency of aberrance （chromosomal bridges, fragments, or micronuclei） was lower, in all 10 taxa compared with other gynmosperms. The meiotic index, average configuration, and frequency of irregularity were found to be uniform among the species. It was shown that the genomes of the Pinus species investigated were highly stable, confirming results of previous mitotic analyses in this genus. However, slight differentiation of homologous chromosomes among genomes was revealed by analysis of meiotic configurations in Pinus nigra var. poiretiana. Quadrivalents were observed in 9.31% of PMCs in this species. This is the first time that quadrivalents have been observed in gymnosperms.The meiotic behavior of 10 taxa （nine species and one variety） of the genus Pinus was investigated using pollen mother cells （PMCs） to reveal the differentiation among karyotypes. Chromosome spreads were prepared by conventional squashing. The meiotic index and the average configuration were higher, whereas the frequency of aberrance （chromosomal bridges, fragments, or micronuclei） was lower, in all 10 taxa compared with other gynmosperms. The meiotic index, average configuration, and frequency of irregularity were found to be uniform among the species. It was shown that the genomes of the Pinus species investigated were highly stable, confirming results of previous mitotic analyses in this genus. However, slight differentiation of homologous chromosomes among genomes was revealed by analysis of meiotic configurations in Pinus nigra var. poiretiana. Quadrivalents were observed in 9.31% of PMCs in this species. This is the first time that quadrivalents have been observed in gymnosperms.

关 键 词：homologous chromosomes IRREGULARITY meioses PINUS pollen mother cell quadrivalent. 

分 类 号：Q343.24[生物学—遗传学] S666[农业科学—果树学]