应用基因芯片技术筛选大鼠再生肝中差异表达基因  

A strategy to identify differentially expressed genes during rat liver regeneration using cDNA array

作　　者：强晖[1] 谢渭芬[2] 施斌[2] 周国雄[1] 倪润洲[1] 

机构地区：[1]南通大学附属医院消化内科,江苏南通226001 [2]上海长征医院消化内科

出　　处：《胃肠病学和肝病学杂志》2009年第7期623-625,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的应用基因芯片技术筛选大鼠再生肝中差异表达基因,为探讨急性肝功能衰竭的发病机制提供基础。方法雄性SD大鼠行95%肝部分切除术,用含1176个基因的大鼠尼龙膜微阵列筛选大鼠再生肝组织中差异表达的基因。RT-PCR随机验证若干差异表达基因在微阵列中的表达。结果再生肝组织有138个基因明显较对照组织表达高,它们主要是生长因子基因、核受体基因、核糖体蛋白基因、应急反应蛋白基因、细胞周期调节基因、神经递质基因等;下调基因共50个,主要为代谢酶基因、激素受体基因及表面抗原基因等。结论cDNA微阵列技术有助于研究急性肝功能衰竭的发病机制,并提供诊断和治疗的潜在靶点。Objective To screen the differentially expressed genes in liver regeneration with eDNA array and to investigate the pathogenesis of acute hepatic failure. Methods Acute liver failure of Sprague-Dawley （SD） rat was induced by 95% hepatectomy. The differentially expressed genes in regenerating rat liver were screened by using a eDNA array representing 1 176 eDNA clusters. Some genes were randomly selected from a pool of differentially expressed genes and subjected to further confirm the result of microarray hybridization by RT-PCR. Results One hundred and thirty- eight genes up-regulated including those of growth factors, PCNA, ribosomal proteins, IL-6 and edks, which were associated with cell cycle regulation, stress, metabolism and proliferation. Fifty genes down-regulated including metabolic enzyme genes, ER genes, surface antigen genes and so on. Conclusion cDNA array is a powerful tool to explore the gene expression of acute liver failure and provides potential targets for the diagnosis and treatment.

关 键 词：基因芯片 大鼠 再生肝 

分 类 号：R515.3[医药卫生—内科学]