慢性疲劳综合征患者外周血单个核细胞中转化生长因子β1 mRNA及雌二醇受体β野生型mRNA的表达水平  

The levels of transforming growth factor-β1 mRNA and estrogen receptor βwt mRNA expression on peripheral blood monocytes of patients with chronic fatigue syndrome

作　　者：张海莹[1] 刘占东[2] 胡朝军[1] 张洋[1] 冯雪[1] 包书萌[1] 张拥波[2] 王得新[2] 李永哲[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院风湿免疫科,100032 [2]首都医科大学附属北京友谊医院神经内科

出　　处：《中华检验医学杂志》2009年第7期745-748,共4页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目（30471617,30640084,30872331）;国家高技术研究发展计划（863计划）资助项目（2002AA222011,2006AA022436）

摘　　要：目的探讨慢性疲劳综合征（CFS）患者外周血单个核细胞（PBMCs）中转化生长因子β1（TGF-β1）mRNA及雌二醇受体13野生型（ERβwt）mRNA表达及其与CFS的发病关系。方法用实时荧光定量逆转录（FQ-RT）-PCR检测63例CFS患者、50例其他疾病患者（疾病对照组）、50名健康体检者（健康对照组）外周血PBMCs中TGF—β1 mRNA和ERβwt mRNA的表达水平和含量。以△Ct=Ct（待测基因）-CT（内参基因）来比较基因表达水平的变化。结果CFS患者TGF—β1和mRNA表达水平（△Ct=3．27±0．58）明显高于健康对照组（△Ct=4．37±1．00，t=7．02，P〈0．01）和疾病对照组（△Ct=4．54±1．05，t=8．11，P〈0．01）。CFS患者ERISwtmRNA表达水平（△Ct=9．34±0．92）明显低于健康对照组（△Ct=7．104-0．48，t=15．47，P〈0．01）与疾病对照组（△Ct=7．12±0．47，t=15．44，P〈0．01）；结论CFS患者TGF-β1mRNA表达水平显著高于健康人和疾病对照组；CFS患者ERIgwt mRNA表达水平显著低于健康人和疾病对照组，TGF-β1 mRNA和ERβ1wt mRNA的表达可能参与了CFS的发病过程。Objective To detect the expression of TGF-β1 mRNA and ERβwt mRNA in peripheral blood mononuclear cells （PBMCs） in patients with chronic fatigue syndrome （ CFS）, and their relationship with pathogenesis of CFS. Methods Fluorescence quantitative RT-PCR （FQ-RT-PCR） was used to examine TGF-β1 mRNA and ERβwt mRNA expression of peripheral blood monocytes in 63 cases with CFS,50 cases with other diseases, and 50 healthy controls. The gene expression levels were calculated with the formula △Ct = Ct（ target gene） -Ct （internal control）. Results The mean TGF-β1 mRNA expression of CFS patients （ △Ct = 3.27 ± 0. 58） was higher than that of disease controls （ △Ct = 4. 54 ± 1.05, t = 8.11, P 〈 0. 01 ） and that of healthy controls （ △Ct = 4. 37 ± 1.00, t = 7.02, P 〈 0. 01 ）. The mean ERβwt mRNA expression of CFS patients （ △Ct =9. 34 ±0. 92） was lower than that of disease controls（ △Ct =7.12 ±0. 47, t = 15.44,P 〈 0. 01 ） and that of healthy controls （ △Ct = 7.10 ±0.48, t = 15.47, P 〈 0. 01 ）. Conclusions The TGF-β1 mRNA and ER βwt mRNA expression levels of PBMCs are significantly elevated in patients with CFS. It may be implicated in the pathogenesis of CSF.

关 键 词：疲劳综合征 慢性 白细胞 单核 转化生长因子Β1 逆转录聚合酶链反应 

分 类 号：R686[医药卫生—骨科学]