HIV 1型B／C重组病毒感染者Nef特异性T淋巴细胞免疫应答的研究  

Analysis of Nef-specific interferon-γ secretion responses in HIV-1 B/C recombinant virus infectors

作　　者：刘宏伟[1] 洪坤学[2] 马军[3] 袁霖[2] 刘沙[2] 陈健平[2] 张远志[3] 阮玉华[2] 王哲[1] 邵一鸣 

机构地区：[1]河南省疾病预防控制中心性病艾滋病研究所,郑州450016 [2]中国疾病预防控制中心艾滋病中心病毒免疫室 [3]乌鲁木齐新疆疾病预防控制中心

出　　处：《中华检验医学杂志》2009年第7期754-759,共6页Chinese Journal of Laboratory Medicine

摘　　要：目的研究中国主要流行的HIV 1型（HIV-1）B／C重组毒株感染者Nef蛋白特异性T淋巴细胞反应特征。方法队列收集19例感染时间〈1年和40例感染时间〉3年的HIV-1 B／C重组毒株感染者，通过酶联免疫斑点试验（Elispot）检测其针对HIV-1 B／C重组毒株Nef重叠多肽产生1干扰素（IFN-1）的特异性T淋巴细胞反应。结果15例感染时间〈1年的HIV-1 B／C重组毒株感染者产生Nef特异性分泌IFN-γ的T淋巴细胞反应，主要识别氨基酸位置为Nef 83至135内的EVA7081．1、EVA7081．5、EVA7081．6、EVA7081．48共4条多肽；29例（75．2％）感染时间〉3年的感染者产生Nef特异性分泌IFN-γ的T淋巴细胞反应，主要识别氨基酸位置为Nef 63至101内的EVA7081．43、EVA7081．44、EVA7081．45、EVA7081．47、EVA7081．48、EVA7081．49共6条多肽。感染时间〈1年的感染者平均反应强度为284．13 SFC／10^6 PBMC，感染时间〉3年的感染者平均反应强度为152．44SFC／10^6 PBMC，2组比较差异具有统计学意义（U=91．000，P=0．002）。结论HIV-1 B／C重组病毒感染者在感染时间〈1年和〉3年时机体均识别HIV-1 Nef的中心区域，其产生IFN-1的特异性T淋巴细胞反应强度随疾病的进展逐渐减小。Objective To analyze characteristics of Nef-specific T lymphocyte responses in Chinese HIV-1 recombinant subtype B/C infectors. Methods 19 HIV-1 recombinant subtype B/C infectors infected within 1 year, 40 chronic infectors infected for more than 3 years were enrolled in this cohort study. Elispot assay was used to observe HIV-1 specific T lymphocyte responses in HIV-1 recombinant subtype B/C infectors. Results Nef-specific T lymphocyte responses of interferon-gamma secretion were identified in 15 Chinese HIV-1 recombinant subtype B/C infectors infected within 1 year. The specific T lymphocytes were mainly targeted at four peptides which span from Nef 83 to 135 ： EVA7081.1, EVA7081.5, EVA7081.6 and EVA7081.48. Responses were identified in 29 （75.2%） infectors with more than 3 years of infection and the specific T lymphocytes were mainly targeted at three peptides which span from Nef 63 to 101 ： EVA7081.43, EVA7081.44, EVAT081.45, EVA7081.47, EVA7081.48 and EVA7081.49. The average magnitude of response in infectors with less than 1 year of infection was 284. 13 SFC/106 PBMC. The average magnitude of response in infectors with more than 3 years of infection was 152.44 SFC/106 PBMC. There was a significant difference between the two groups （ U = 91. 000, P = 0. 002 ）. Conclusions HIV-1 recombinant subtype B/C infectors at different stages of diseases （less than 1 year and more thank 3 years） can recognize central region of Nef. The magnitude of Nef-specific IFN-γ secretion T lymphocyte responses in this cohort gradually decrease with disease progression. Interferon type Ⅱ

关 键 词：HIV感染 HIV-1 nef基因产物 人免疫缺陷病毒 T淋巴细胞 干扰素Ⅱ型 

分 类 号：R686[医药卫生—骨科学]