唾液酸结合免疫球蛋白样凝集素1在冠状动脉粥样硬化性心脏病患者外周血单个核细胞的表达及临床意义  

The expression of sialic acid-binding inununoglobulin-like lectin 1 on peripheral mononuclear cells in patients with coronary heart disease and its clinical significance

作　　者：熊怡淞[1] 周运恒[1] 吴炜霖[1] 张玲珍[1] 梁艳[1] 杨再兴[1] 耿红莲[1] 王皓[1] 王爱华[1] 荣光华[1] 仲人前[1] 

机构地区：[1]第二军医大学长征医院实验诊断科全军医学免疫诊断中心全军临床免疫重点实验室,上海200003

出　　处：《中华检验医学杂志》2009年第7期772-776,共5页Chinese Journal of Laboratory Medicine

基　　金：国家高技术研究发展计划（“863”计划）资助项目（2006AA022496）;上海市科学技术委员会优秀学科带头人基金资助项目（07XD14013）

摘　　要：目的观察唾液酸结合免疫球蛋白样凝集素1（sialic acid—binding immunoglobulin—like lectin 1，Siglec．1，也称CD169）在冠状动脉粥样硬化性心脏病（CHD）患者外周血淋巴细胞、单核细胞和中性粒细胞上的表达水平，并探讨其与冠状动脉粥样硬化发生发展的关系。方法流式细胞术检测57例CHD患者及38名健康对照者外周血CD14,CD169双阳性细胞的表达率；生化常规测定所有入选者血脂水平；实时荧光相对定量逆转录（FQ—RT）-PCR方法检测入选对象外周血单个核细胞（PBMCs）中CD169 mRNA的含量。结果流式细胞仪检测发现，CD169在健康对照组及CHD组淋巴细胞和中性粒细胞上均无表达；CHD组单核细胞CD14CD169双阳性率为（12．7±2．4）％，显著高于健康对照组[（1．0±0．3）％，t＝23．2，P〈0．01]；CHD组CD169 mRNA的拷贝数显著高于健康对照组拷贝数（t=6．59，P〈0．01），为健康对照组的3．2倍；而CHD血脂正常组和血脂异常组的CD169阳性率[分别为（12．2±2．3）％和（13．4±2．5）％，f=1．87，P〉0．05]和mRNA平均拷贝数（分别为健康对照组的3．64和2．79倍，t=0．98，P〉0．05）差异均无统计学意义。结论CD169组成性表达于特定组织巨噬细胞上，健康人外周血单核细胞上并不表达，单核巨噬细胞受到炎症刺激时，CD169表达迅速上调。CD169蛋白及mRNA含量在CHD患者外周血单核细胞上表达显著升高，CHD患者外周血单核细胞已发生巨噬细胞化，单核巨噬细胞介导的免疫炎症反应在CHD发生发展过程中起重要作用。Objective To investigate the expression of sialic acid-binding immunoglobulin-like lectin-one （Siglee-1, also called CD169 ） in lymphocytes, monoeytes and neutrophils in peripheral blood in patients with coronary heart disease（CHD）, and explore the relationship between Siglec-1 expression and atherosclerosis. Methods CD14 CD169 positive cell proportion and CD169 mRNA levels were respectively measured by flow cytometry and real-time quantitative reverse transcription-polymerase chain reaction （ FQ- RT-PCR） in 57 CHD patients and 38 healthy controls. And the levels of serum lipids were determined by automatic biochemistry analyzer. Results The flow cytometry analysis showed that CD169 protein was not found in lymphocytes and neutrophils in both CHD patients and healthy controls. The rate of CD14 CD169 double positive cells in monocytes in CHD group was significantly higher than that in healthy controls [ （12. 7 ± 2. 4）% vs （1.0 ±0. 3 ）% ,t = 23.2, P 〈 0. 01 ]. And FQ-RT-PCR analysis showed that the mean CD169 mRNA copy number in PBMCs in CHD group was significantly higher（ 3.2 fold） than that in healthy controls [ t = 6. 59 ,P 〈 0. 011. However, neither differences of CD169 protein positivities [ [ （ 12. 2 ± 2. 3 ） % vs （ 13.4± 2. 5 ） %, t = 1.87, P 〉 0. 05 ] nor mRNA levels [ 3.64 fold vs 2. 79 fold when compared with healthy controls,t = 0. 98, P 〉 0.05 ] were found between CHD patients with normal and abnormal levels of serum lipids. Conclusions CD169 is mainly expressed in human tissue-resident macrophages but not expressed in peripheral blood monocytes. And when the monocytes is stimulated by inflammation, the expression of CD169 is increased. In patients with CHD, the increased expression of CD169 protein and mRNA level has demonstrated the activation of monocytes in peripheral blood. CD169 and CD169-mediated monocytes activation may play an important role in the development and progression of atherosclerosis.

关 键 词：冠心病 白细胞 单核 膜糖蛋白类 受体 免疫 流式细胞术 

分 类 号：R686[医药卫生—骨科学]