Acidithiobacillus ferrooxidans ATCC23270与矿物吸附过程的热量变化及不同条件下代谢热的基础研究  

Study on the Enthalpy Variation during Adsorption Processes of Acidithiobacillus ferrooxidans ATCC23270 on the Surface of Minerals and Its Metabolic Thermogenesis under Different Conditions

作　　者：王秀美[1,3] 柳建设[2] 李邦梅[1] 朱建裕[1] 王铧泰[1] 闫颖[1] 邱冠周[1] 

机构地区：[1]中南大学资源加工与生物工程学院,长沙410083 [2]东华大学环境科学与工程学院,上海201620 [3]长春黄金研究院,长春130012

出　　处：《高校地质学报》2009年第2期256-262,共7页Geological Journal of China Universities

基　　金：国家创新研究群体科学基金(50621063);国家973基础研究项目(2004CB619201);全国优秀博士论文作者专项资金资助项目(200549);国家自然科学基金(30700008)联合资助

摘　　要：用微量热技术研究了不同条件下Acidithiobacillus ferrooxidans ATCC23270（以下简写为A.ferrooxidans ATCC23270）与硫化矿吸附过程的热量变化以及不同细菌浓度、不同的初始pH值及不同的培养条件下细菌的代谢产热情况。研究发现,矿浆浓度0.03g/mL,细菌浓度1.7×10^8个/mL的时候细菌与矿物的吸附放热最大。不同条件培养的细菌胞外多聚物的组成不同,与矿物吸附的反应热也不相同,黄铁矿培养细菌胞外多聚物含量最高,反应热也最高,说明细菌胞外多聚物在吸附过程中起重要作用。用微量热法能够很好地反映出细菌生长代谢过程中每个微观时刻的热量变化。对于A.ferrooxidans ATCC23270,最佳的代谢产热条件为：pH值为2.0和2.3,细菌浓度为3.0×10^8个/mL。不同培养条件下的细菌的生长代谢热不同,2039＋FeSO4培养的代谢热最大、放热最快。The influencing factors of the enthalpy variation during adsorption processes of Acidithiobacillus ferrooxidans ATCC23270 on the surface of sulfide minerals and the metabolic thermogenesis of the bacteria under different conditions （different concentrations of the bacteria, different initial pH values and different media） were studied using microcalorimetry. The results show that the concentration of pulp at 0.03 g/mL and concentration of bacteria at 1.7 × 10^8 were the best environment for enthalpy variation during adsorption processes of A. ferrooxidans ATCC23270 on the surface of minerals. A. ferrooxidans cultured in different media with different contents of extracellular polymers （EPS） create different heat of reaction attaching on the surface of minerals. Bacteria cuhured with pyrite having the highest EPS create the biggest heat of reaction, which proves the important role of EPS in the process of adsorption. The growth thermograms of bacteria can be determined by using the mircocalorimetric method. These perfect thermogram curves reflect the changes of bacterial growth patterns. For A. ferrooxidans ATCC23270, the best condition for the metabolic thermogenesis is at pH = 2.0 or 2.3, and concentration of bacteria at 3.0 ×10^8 cell/mL. The bacteria cuhured in different media create different growth thermograms. Media of 2039＋ FeS04 is the best one for metabolic thermogenesis.

关 键 词：微量热技术 A.ferrooxidans ATCC23270 吸附 代谢热 

分 类 号：Q939[生物学—微生物学]