栀子甙对血管性痴呆大鼠行为学和脑组织病理改变的影响  

Effect of Geniposide on Vascular Dementia in Rats

作　　者：李利娟[1] 李宏霞[2] 吴欣桐[1] 鄢波[1] 周东[1] 

机构地区：[1]四川大学华西医院神经内科,成都610041 [2]四川大学华西医院国家成都中药安全性评价中心

出　　处：《四川大学学报（医学版）》2009年第4期604-607,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的观察栀子甙对血管性痴呆(VaD)大鼠认知功能的影响。方法采用双侧颈总动脉结扎制备VaD大鼠模型,予VaD大鼠不同剂量栀子甙〔25mg/(kg·d)、50mg/(kg·d)、75mg/(kg·d)〕灌胃,连续治疗4周后,进行水迷宫实验和脑组织病理检查,结果与假手术组、模型组及多奈哌齐治疗组相比较,观察栀子甙对血管性痴呆大鼠的影响。结果模型组较假手术组大鼠逃避潜伏期延长、平台象限游程/总游程下降(P<0.05);栀子甙高、中剂量治疗组逃避潜伏期短于模型组、平台象限游程/总游程大于模型组(P<0.05),接近于假手术组和多奈哌齐组水平(P>0.05),栀子甙低剂量组与模型组比较未见明显差异,但反映出其具有改善认知损害作用趋势。病理检查显示与模型组相比,栀子甙治疗组大鼠皮层及海马神经元的凋亡坏死明显减轻。结论双侧颈总动脉结扎致慢性低灌注能显著影响大鼠的认知功能,栀子甙对血管性痴呆大鼠模型的认知功能损害有防治作用,具有剂量依赖性。Objective To investigate the effect of geniposide on vascular dementia （VaD） in rats. Methods VaD rat model was established by permanent ligation of bilateral common carotid arteries. Morris water maze performance was assessed after 4 weeks of treatment with geniposide, followed by pathological examinations of hippoeampus and cerebral cortex. Results The VaD rats had significantly longer escape latency and lower percentage of activities in platform quadrant than the rats in the sham surgery group （P〈0. 05）. The VaD rats treated with geniposide [50 mg/（kg · d）, 75 mg/（kg · d）]had significantly shorter escape latency （except the first day of the test） and significantly higher percentage of activities in platform quadrant than the VaD rats without treatment （P〈0.05）. No significant differences were found between the geniposide treated group and the Donepezil treated group and the sham surgery control group. Geniposide significantly alleviated neurons, apoptosis and necrosis induced by chronic cerebral hypoperfusion of cortex and hippocampus. Conclusion Chronic cerebral hypoperfusion can induce cognitive impairment. Geniposide can improve cognitive ability of Vascular Dementia in rats. This may represent a potential treatment strategy for vascular dementia.

关 键 词：栀子甙 血管性痴呆 学习和记忆 

分 类 号：R285.5[医药卫生—中药学]