脂联素改善肥胖大鼠离体主动脉内皮依赖性血管舒张功能的实验研究  

Effect of Adiponectin on Endothelial Dysfunction in Obese Rats

作　　者：李明锐[1] 邓刚[1] 余叶蓉[1] 

机构地区：[1]四川大学华西医院内分泌科,成都610041

出　　处：《四川大学学报（医学版）》2009年第4期612-614,627,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30570874)资助

摘　　要：目的研究脂联素对食物诱导的肥胖大鼠内皮依赖性血管舒张功能的影响。方法将10只SD雄性大鼠分为2组,正常对照组予普通饲料喂养;高脂饲料组予高脂饲料喂养。6周后处死动物,将3mm长的主动脉环置于培养皿中,分别以脂联素及脂联素加内皮一氧化氮合成酶特异性抑制剂(L-NAME)孵育离体血管环,并与空白对照比较,在器官浴槽中观察离体胸主动脉环对乙酰胆碱或硝普钠的舒张反应。结果高脂饲料喂养的大鼠内皮依赖性舒张功能明显受损,其最大舒张程度仅为对照组的29%。脂联素组内皮依赖性血管舒张功能较高脂饲料组增加102%,脂联素加L-NAME组内皮依赖性血管舒张功能接近丧失。结论脂联素对肥胖动物受损的血管内皮细胞具有确切的直接保护作用。Objective To test the efffect of Adiponectin on endothelium-dependent and non-dependent vasodilation in food-induced obese SD rats. Methods Ten male Sprague-Dawley rats were divided into 2 groups： the rats in the control group （n=5） were fed with normal chow; The rats in the experimental group were fed with high fat chow （n=5）. At the end of the sixth week, all rats were killed and the thoracic aortas were harvested. The vessel rings obtained from the obese rats were pre-incubated for 2 hours with adiponectin, or adiponectin plus LNAME, or none of them, then equilibrated in Krebs-Henseleit solutions. Constriction of the vessel rings were induced by norepinephrine followed by relaxations with increasing doses of acetylcholine （for endothelium dependent vasodilatation, EDV） or sodium nitroprusside （for endothelium independent vasodilation, EIV）. Vasorelaxation was expressed as percentage of residual of maximal contraction. Results Acetylcholine （10^-8-10^-4 mol/L） caused a concentration-dependent relaxation in all preconstrictcd aortic rings. Compared with the control group, the maximal endothelium dependent relaxation of the aortic rings in the obese rats was impaired （p〈0.05）. With A.diponectin, the relaxation response of aortic rings to acetylcholine improved （P〈0. 05）. Sodium nitroprusside （10^-8-10^-4 mol/ L） induced relaxation （endothelium independent relaxation） did not differ statistically between the two groups. Conclusion Impaired endothelium-dependent vasodilatation has been observed in food-induced obese rats and Adiponectin can improve the endothelial dysfunction in obese rats.

关 键 词：肥胖 脂联素 内皮依赖性血管舒张功能 

分 类 号：R589.2[医药卫生—内分泌]