ERK及STAT3在机械力刺激肺泡上皮A549细胞信号传导中的作用  

Effects of ERK and STAT3 on Cell Signal Transduction in Alveolar Epithelium A549 Cells Stimulated by Mechanical Force

作　　者：安静[1] 韩素霞[2] 侯艳[1] 周虹[1] 汪涛[1] 徐丹[1] 胡小容[1] 文富强[1] 

机构地区：[1]四川大学华西医院呼吸内科呼吸病学研究室,成都610041 [2]新疆医科大学第五附属医院心内科

出　　处：《四川大学学报（医学版）》2009年第4期636-638,650,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30425007;30370627;30670921);美国纽约CMB基金(00-722;06-834);中国博士点基金(20060610064)资助

摘　　要：目的研究机械张应力对肺泡上皮A549细胞MAPK信号通路蛋白ERK和JAK/STAT通路蛋白STAT3丝氨酸位点磷酸化水平表达的影响。方法利用自制四点弯曲加力装置对A549细胞进行大小为3000μstrain的张应力刺激,收集不同时间点(30min,1h,2h,4h)的细胞总蛋白,用Western blot的方法检测磷酸化STAT3和ERK蛋白水平的变化。设未予机械力刺激的A549细胞为对照组。结果①用3000μstrain的张应力刺激A549细胞后,p-ERK表达从1h开始增加,和对照组相比差异有统计学意义(P<0.01)。②用3000μstrain的张应力刺激A549细胞后,p-STAT3(Ser)表达随时间增加,与对照组相比差异有统计学意义(P<0.01)。结论MAPK家族中的ERK和STAT家族中的STAT3可能参与了机械力刺激肺泡上皮细胞的信号传导。Objective To observe the effect of mechanical force on the expression of p-ERK and p-STAT3 when pulmonary epithelium A549 cells were subjected to tensile stress. Methods By the self-made four-point bending system, the A549 cells were subjected to cyclic tensile stress of 3000 μstrain for 30 rain, 1 h, 2 h or 4 h respectively, then the total protein of each time point was collected and the expression of p-ERK and p-STAT3 were detected by Western blot analysis. Results （1）The expression of p-ERK was activated at 1 h and remained at a high level until 4 h. （2）The expression of p-STAT3（Ser） was activated at 30 min and remained at a high level until 4 h. Conclusion Tensile stress can activate the expression of p-ERK and p-STAT3 （Ser） in alveolar epithelium A549 cells. ERK and STAT3 may participate in the cell mechanotransduetion in A549 cells.

关 键 词：机械张应力 力学信号传导 A549细胞 ERK STAT3 

分 类 号：R459.6[医药卫生—治疗学]