生物素化抗AIB1单克隆抗体的制备及应用研究  

Preparation and Application of Biotinylated Anti-AIB1 Moniclonal Antibody

作　　者：庞秋霞[1] 屈艺[2] 魏大鹏[3] 赵凤艳[2] 

机构地区：[1]延安大学医学院生物化学与分子生物学教研室,延安716000 [2]四川大学华西第二医院儿科 [3]四川大学华西基础医学与法医学院免疫学研究室

出　　处：《四川大学学报（医学版）》2009年第4期727-729,共3页Journal of Sichuan University(Medical Sciences)

基　　金：陕西省教育厅科研基金(编号:08JK500)资助

摘　　要：目的用生物素N-羟基丁二酰亚胺酯(BNHS)对纯化抗AIB1单克隆抗体1A2E1进行标记,并应用标记后单克隆抗体对靶细胞中AIB1蛋白进行检测。方法用BNHS与纯化抗体1A2E1交联,制备Biotin-1A2E1,用竞争抑制ELISA法及免疫细胞化学法检测Biotin-1A2E1的生物学活性。结果所制备的生物素化抗体活性高,效价为1∶3200,用于检测乳腺癌细胞中AIB1蛋白敏感性好。结论成功制备生物素化抗体Biotin-1A2E1,并可初步应用于靶细胞AIB1蛋白的检测,为肿瘤辅助诊断中AIB1蛋白的检测提供有力手段。Objective To cross-link the McAb 1A2 E1 to BNHS reagents and apply this biotinylated McAb to detect AIB1 antigen of target cells. Methods The McAb 1A2E1 was mixed with BNHS reagents to generate Biotin- 1A2E1. The competitive inhibition ELISA and immunocytochemical method were applied to detect the biologic activity of antibody. Results The antibody kept high biological activities （with a titer of 1 ： 3200） and sensitivities in detecting the AIB1 protein of breast cancer cell. Conclusion The method of preparing biotinylated McAb is successful, and the prepared biotinylated McAb can be applied to detect target cells which express AIB1 antigen. This McAb provide useful tool for tumor auxiliary diagnosis.

关 键 词：生物素 N-羟基丁二酰亚胺酯 生物素化 单克隆抗体 AIB1 

分 类 号：R392-33[医药卫生—免疫学]