温石棉与吸烟联合作用于人胚肺细胞时对活性氧产生的影响  

The combined effect of chrysotile and cigarette smoking on the production of reactivated oxygen species in human embryo lung cells

作　　者：王起恩[1,2] 樊晶光[1,2] 卢景芬[1,2] 刘宏宇[1,2] 刘世杰[1,2] 

机构地区：[1]北京医科大学劳动卫生教研室 [2]北京医科大学天然与仿生药物国家重点实验室

出　　处：《卫生毒理学杂志》1998年第2期82-85,共4页Journal of Health Toxicology

摘　　要：为探讨温石棉（ＣＨ）与吸烟联合作用于人胚肺（ＨＥＬ）细胞时对反应体系中活性氧产生的影响，本研究采用不同剂量的ＣＨ和香烟烟溶液（ＣＳＳ）单独及联合作用于ＨＥＬ细胞，观察了Ｈ２Ｏ２、Ｏ—·２和·ＯＨ的产生情况。结果表明，ＣＨ和ＣＳＳ单独与ＨＥＬ细胞作用２小时，反应体系中Ｈ２Ｏ２的产生均明显升高，当不同剂量的ＣＨ和ＣＳＳ联合作用时Ｈ２Ｏ２的增加呈相加或加强作用；ＣＳＳ与ＨＥＬ细胞单独作用１小时，反应体系中Ｏ—·２也明显升高，当ＣＨ与ＣＳＳ同时作用时对Ｏ—·２的增加呈相加作用；用ＥＳＲ直接测定反应体系中·ＯＨ的产生情况表明ＣＨ和ＣＳＳ可协同增加·ＯＨ的产生。In order to investigate the combined effect of chrysotile and cigarette smoking on the production of reactivated oxygen species in human embryo lung(HEL) cells,the production of H 2O 2, O ·- 2 and OH were determined in HEL cells which were incubated with chrysotile(CH) and cigarette smoking solution(CSS) separately or simultaneously.The results showed that H 2O 2 increased significantly when HEL cells were incubated with CH or CSS separately,O ·- 2 increased significantly when HEL cells were incubated with CSS singly, and ·OH increased when HEL cells were incubated with CH singly.All H 2O 2, O ·- 2 and ·OH increased synergistically when HEL cells were exposed to CH and CSS simultaneously. \ \

关 键 词：温石棉 吸烟 活性氧 人胚肺 细胞 

分 类 号：R135.2[医药卫生—劳动卫生]