白腐真菌产锰酶及对酸性蓝45的脱色  

DECOLORIZATION OF ACID BLUE 45 BY MNP PEROXIDASE OF PHANEROCHAETE CHRYSOSPORIUM

作　　者：连小英[1,2] 于丹[2] 周成[1] 赵海燕[2] 文湘华[1] 

机构地区：[1]清华大学环境科学与工程系环境模拟与污染控制国家联合重点实验室,北京100084 [2]长安大学环境科学与工程学院,陕西西安710054

出　　处：《水处理技术》2009年第7期34-37,50,共5页Technology of Water Treatment

摘　　要：为提高白腐真菌在自由悬浮条件下锰过氧化物酶(MnP)的产量,在培养液中投加打结棉线载体,通过载体投加量和培养条件的调整,在载体投加量370g·L-1、转速130r·min-1、温度37℃,葡萄糖和酒石酸氨的质量浓度分别为10g·L-1和0.8g·L-1条件下,MnP产量从自由悬浮培养的7.10提高到24.5mkat,初始MnP也提前3d出现。以370g·L-1载体投加体系为例,考察其对不同含量酸性蓝45的脱色效果,2d内各含量染料的脱色率均超过94%,同时该体系对酸性蓝45具有一定重复脱色能力,重复脱色次数与染料的含量有关。The mobilized and immobilized conditions were compared to obtain a high MnP productivity. The results showed that the optimum conditions were： the temperature of the shaking table 37℃, rotating speed 130 r·min^-1,carrier content 370g·L^-1, glucose concerntration 10 g·L^-1, ammonium tartrate 8.0 g·L^-1 .When cultured in the optimum condition the highest productivity of MnP was 1 469 U/L, and the decolorization rates of acid blue 45 in the optimum system were all over 94% in 2 days, after 2 days the system still had decolorization capacity.

关 键 词：白腐真菌 锰过氧化物酶 固定化 染料 脱色 

分 类 号：X788[环境科学与工程—环境工程]