大豆蛋白质相关基因SNP的开发和遗传定位  

SNP discovery and linkage analysis of protein related genes in Soybean

作　　者：蔡春梅[1] 柳伟先 李锡河 

机构地区：[1]青岛农业大学生命科学学院,山东青岛266109 [2]韩国首尔大学农业与生命科学学院,韩国首尔151921

出　　处：《延边大学农学学报》2009年第2期77-83,共7页Agricultural Science Journal of Yanbian University

基　　金：韩国科技部支撑项目(CG3121)

摘　　要：在大豆蛋白质相关基因中利用直接测序法在2个代表性品种Pureukong与Jinpumkong 2之间发掘SNP标记,并利用这些标记将蛋白质相关基因定位在遗传图谱上.为了开发SNP分子标记,选择56个蛋白质相关基因并设计相应的引物.结果显示,49个引物获得较好的测序结果.其中,7个引物在2个亲本之间显示多态性,共检测出11个SNP分子标记.2个亲本间SNP的检测频率为1 SNP/2400 bp.利用杂交获得的重组自交系,大豆蛋白质相关基因被定位到已经构建的以SSR分子标记为主的遗传图谱上.Found SNPs between Purenkong and Jinpumkong 2 using direct sequencing method and to map these genes to soybean linkage map. To discover SNPs, a total of 76 protein related genes were selected and primers were designed. Among 49 loci showing high-quality sequence, 11 SNPs were identified in 7 loci between the parental genotypes. Average SNP frequency between the parental genotypes was one SNP per 2 400 bp. With SNP genotyping in 90 RILs, these genes were mapped in exiting linkage map.

关 键 词：大豆 SNP 蛋白质 

分 类 号：S565.1[农业科学—作物学]