二次回归通用旋转设计优化土茯苓总黄酮的提取工艺  

Optimization of the Extraction Technique of Total Flavonoids from Smilax Glabra Roxb. by Quadratic Regression General Rotary Unitized Design

作　　者：夏道宗[1] 于新芬[2] 马志杰[1] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]杭州市疾病预防控制中心,浙江杭州310006

出　　处：《中华中医药学刊》2009年第7期1460-1463,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(Y207783)

摘　　要：目的:采用二次回归通用旋转试验设计优化土茯苓总黄酮(TFSG)的提取工艺。方法:根据Box-Be-hnken的中心组合试验设计原理,在单因素试验的基础上采用四因素三水平的响应曲面分析法,依据回归分析确定各工艺条件的影响因子,以TFSG得率(%)为响应值作响应面和等高线图。结果:TFSG超声波辅助提取效果的影响因素由大到小顺序为:乙醇浓度>超声功率>超声时间>料液比。优化后的工艺参数为:乙醇浓度57%,超声功率500W,超声时间34min,料液比1:21,响应值达到2.32%。结论:本文所建立的数学模型极其显著(P<0.01),可用来对TFSG的提取工艺进行分析和预测。Objective：To optimize the extraction technique of total flavonoids from Smilax glabra Roxb. （TFSG） by quadratic regression general rotary unitized design. Methods ：The method of response surface analysis with 4 factors and 3 levels was adopted according to the Box-Behnken center-united experimental design prineiples and one-factor tests. Then the factors influencing the technological parameters were determined by means of regression analysis. Response surface and contour were finally graphed with the extraction rate of TFSG（ % ）as the response value. Results ：The factors which influence the extraction rate of TFSG aecording to priority was ethanol concentration,ultrasonic power, ultrasonic extraction time, and solid/llquid ratio. The optimum eunditions of TFSG extraction were： the ethanol concentration of 57%, the ultrasonic power of 500 W,the ultrasonic extraction time of 34 rain,the solid/liquid ratio of 1：21 ,and the extraction rate of TFSG was up to 2. 32 %. Conclusion： The mathematical model that the present study established was significant （ 19 〈 0.01 ） ,and it might be applied to analysis and prediction the extraction technique of TFSG.

关 键 词：二次回归通用旋转设计 土茯苓 总黄酮 提取工艺 

分 类 号：R284.1[医药卫生—中药学]