猕猴桃根抗肝癌的实验研究  

Anti-tumor Activity of Actinidia Chinensis Planch Against Hepatocellular Carcinoma

作　　者：楼丽君[1] 吕定量[1] 胡增仁[1] 胡泽富[1] 郑方平[1] 

机构地区：[1]衢州市人民医院,浙江衢州324000

出　　处：《中华中医药学刊》2009年第7期1509-1511,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：衢州市科技计划基金资助项目(20041998)

摘　　要：目的:评价猕猴桃根提取物的体外、内抗肝癌作用。方法:猕猴桃根活性成分的分离、纯化采用传统的天然产物化学方法;不同组分对体外培养的肝癌Bel-7402细胞作用采用磺酰罗丹明B方法;猕猴桃根的体内抗肿瘤作用采用小鼠肿瘤和人肿瘤裸小鼠移植瘤模型评价。结果:猕猴桃根先用甲醇回流提取,然后用乙酸乙酯、氯仿、正丁醇萃取。氯仿提取物对肿瘤细胞增殖抑制作用最强,甲醇提取物次之。体内实验证实氯仿提取物有效地抑制小鼠肝癌模型和人肝癌裸小鼠移植瘤模型的生长,抑制率大概在38.0%。结论:猕猴桃根提取物有一定的抗肿瘤作用;其活性成分主要存在于极性较小的组分。Objective：To evaluate the in vitro and in vivo anti-tumor activity of the extract from the root of Actinidia chinensis planch against hepetocellular carcinoma cells. Methods：Active components from the root of Actinidia chinensis planch were isolated by traditional phytochemical techniques. The in vitro anti-tumor activity was determined by sulforhodamine B assay and the in vivo anti-tumor activity was evaluated using experimental mouse tumor models and human tumor xenografts in nude mice. Results ： Powdered air-dried roots of Actinidia chinensis planch were percolated with methanol at room temperature thrice. The filtrate was concentrated to dryness in vacuo and then was further extracted with ethyl acetate, n-butanol, and chloroform. The fraction extracted by chloroform displayed potent activity against hepatocellular carcinoma Bel -7402 ceils. Further more ,the anti-tumor efficacy of the chloroform fraction was confirmed in murine hepatocellular carcinoma H,, and human Be1-7402 xenografts. Conclusion ：The extract of the root of Actinidia chinensis planch has anti-hepatocellular carcinoma activity ,and the active components is mainly in the fracdon extracted by chloroform.

关 键 词：猕猴桃根 藤梨根 肝癌 中药药理 

分 类 号：R285.5[医药卫生—中药学] R730.52[医药卫生—中医学]