鸡传染性支气管炎病毒地方流行株的分离与鉴定  

Isolation and biological properties of avian infectious bronchitis virus isolated from Shanxi province

作　　者：闫芳[1] 岳文斌[1] 刘娟[1] 李绪英[1] 赵宇军[1] 吉文汇[1] 刘风波[1] 吴倩[1] 任家琰[1] 化丽珍[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国兽医学报》2009年第7期845-848,共4页Chinese Journal of Veterinary Science

基　　金：山西省自然科学基金资助项目(20070110830);山西高校科技研究开发资助项目(200611014)

摘　　要：从山西各地区疑似鸡传染性支气管炎（IB）的病料中，分离到5株鸡传染性支气管炎病毒（IBV）分离株，并对分离病毒进行了病毒形态观察、对鸡新城疫病毒（NDV）的干扰、鸡胚致病性试验、动物回归试验、血凝特性试验、病毒理化特性测定等生物特性鉴定及IBVN基因特异性片段的检测。电镜观察，可见直径为60～120nm，有囊膜及纤突呈冠状排列的病毒粒子；对NDV有明显的干扰作用；分离株的传代物均有明显的致鸡胚矮小化作用；动物回归感染死亡鸡肾脏病变明显，表现肾脏肿大、花斑肾现象，输尿管内充塞大量尿酸盐；无直接血凝性，经1％胰酶处理后可凝集鸡红细胞；分离株对乙醚和氯仿敏感；采用反转录-聚合酶链式反应（RT—PCR）对分离毒株进行扩增，结果均扩增出特异N基因核酸片段。Five field strains of infectious bronchitis virus （IBV） were isolated from suspected flocks from different time and different regions of Shanxi province,respectively,and characterized by a series of systematic identification assays,such as morphological observation by electron-microscope,interfering with the propagation of NDV, virus pathological role to chicken embryo,virus pathological role to SPF chickens,hemagglutination activity,physisco- chemical,and reverse transcription polymerase chain reaction（RT-PCR）. The results showed：The typical coronavirus which the spherical virions 60-120 nm in diameter and surface covered with spike like corona were observed under electron-microscope;The propagation of NDV strain was seriously interfered by the 5 isolates respectively; The embryonated chicken egg passages of the 5 isolates could dwarf with chicken embryos;The five isolates had no hemagglutination activity,but after treatment with 1% trypsin, it can agglutinate chicken red blood cell. The strains are sensitive to chloroform and ethyl ether. The SPF chickens which inoculated with the 5 isolates showed clinical sign and result in respiratory and kidney diseases, flower-steak, and swollen with severe urate deposition. The specific fragments of N gene of the 5 isolates were amplified by RT-PCR, respectiveiy. On the basis of all above mentioned results,the 5 isolates were classified as IBV. The study got a good preparedness for further study on molecular epidemioogy of the 5 IBV isolates.

关 键 词：鸡传染性支气管炎病毒 分离 鉴定 

分 类 号：S852.65[农业科学—基础兽医学]