东北地区猪群链球菌分离鉴定及流行病学调查分析  

Isolation,identifiation and epidemiological analysis of swine Streptococcus in the northeast region of China

作　　者：王淑杰[1] 雷连成[2] 徐敏[1] 孙长江[2] 李成君[1] 蔡雪辉[1] 刘永刚[1] 张琦[1] 刘狄萩 石文达[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2009年第7期877-881,共5页Chinese Journal of Veterinary Science

基　　金：国家“973”计划资助项目(2006CB504401)

摘　　要：为了解猪链球菌在东北地区正常猪群中的流行情况,对黑龙江、吉林、辽宁省等地无菌采集的2 204份鼻拭子接入含有1‰抗生素的链球菌液体选择培养基中,37℃静止培养24 h,挑取细菌培养物涂片,革兰氏染色后镜检,将镜检为革兰氏阳性的链球菌利用PCR方法进行猪链球菌种的鉴定,在此基础上再利用PCR方法进行猪链球菌1、2、7、9血清型的分型鉴定。结果显示,黑龙江、吉林、辽宁3省猪群链球菌的携带率分别为29%、27%、34%,东北地区分离得到猪链球菌共155株,其中1型猪链球菌7株,2型猪链球菌39株,7型猪链球菌4株,9型猪链球菌11株,其他型猪链球菌94株。In order to know that Streptococcus exist in health swine herd, 2 204 cases of nose swabs were collected in swine from different areas in Dongbei three province of northeast China. Seed swab to Streptococcus culture me- dium,24 hours after culturing,gram staining with hecteria culture,gram positive Streptococcus were identified with PCR technology, then farther identified serotype with Streptococcus suis 1,2,7,9 serotype special primers . The results showed that Streptococcus exist rate in swine herd of Heilongjiang,Jinlin, Liaoning is 29 %, 27 %, 34 %, respectively. PCR typing assay indicated that 155 strains of Streptococcus suis were isolated including 7 strains of 1 serotype,39 strains of 2 serotype,4 strains of 7 serotype, 11 strains of 9 serotype, 94 strains of other serotype in three province of northeast China.

关 键 词：猪链球菌 分离鉴定 流行病学调查 

分 类 号：S852.611[农业科学—基础兽医学]