响应面法优化野菊花多糖含量测定的前处理条件  

Pre-treatment in determining total polysaccharide in Flos Chrysanthemi Indici by response surface design

作　　者：房海灵[1] 郭巧生[1] 申海进[1] 

机构地区：[1]南京农业大学中药材研究所,江苏南京210095

出　　处：《中国中药杂志》2009年第13期1665-1667,共3页China Journal of Chinese Materia Medica

基　　金：国家科技基础条件平台项目(2005DKA21000)

摘　　要：目的:优化野菊花多糖含量测定的前处理条件。方法:研究影响野菊花多糖含量测定中前处理条件的几个因素,包括提取温度、提取时间、料液比,并通过响应面分析法优化前处理条件。结果:当提取温度为81.0℃,提取时间为1.6 h,料液比为1∶29时,野菊花多糖含量最高。结论:通过响应面分析法可以得到一个较好预测实验结果的模型方程。Objective： To study pre-treatment in determining total polysaecharide in flos Chrysanthemi Indici. Method： The factors including the extraction temperature, extraction time, ratio of material to liquid were studied. The best extraction condition was found through the response surface design. Result： The best extraction condition as follows： 81.0℃ of the extraction temperature, 1.6 h of extraction time, and the ratio of material to water as 1： 29. On these conditions the extraction rate of flos Chrysanthemi Indici was the best. Conclusion： A model equation that can be used to predict the experiment is established through the response surface method.

关 键 词：野菊花 多糖 响应面分析 

分 类 号：R284.1[医药卫生—中药学]