甘草酸对大鼠体内芍药苷药代动力学的影响  

Influence of glycyrrhizin on paeoniflorin pharmacokinetic fate in unrestrained conscious rats by intravenous administration

作　　者：李娜[1,2] 陈西敬[1] 韩德恩[1] 于雪[1] 苏云明[2] 

机构地区：[1]中国药科大学药物代谢动力学研究中心,江苏南京210009 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中国中药杂志》2009年第13期1720-1723,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30472060);国家高技术研究发展计划(863)项目(2007AA02Z171)

摘　　要：目的:建立一种简单、快速的HPLC方法用于研究在大鼠体内甘草酸对芍药苷药代动力学特性的影响。方法:采用反相高效液相色谱及内标法测定大鼠血浆中芍药苷浓度,实验动物分为对照组和实验组,对照组单独给予芍药苷,实验组给予芍药苷和甘草酸,尾静脉注射给药,应用WinNonlin软件以非房室模型计算药代动力学参数。结果:AUC(min.mg.L-1),Vd(mL.kg-1)、CL(mL.min-1.kg-1)对照组分别为166.81±26.94,394.33±29.52,18.40±3.12,实验组分别为235.44±46.48,266.63±48.43,13.16±2.59。结论:静注甘草酸可以增加芍药苷的AUC,降低CL,减小Vd(P<0.05),对其他药代动力学参数没有影响,推测可能是影响了其代谢和排泄。Objective： The aim of this study is to develop a simple and rapid HPLC method and investigate the effect of glycyr- rhizin on pharmacokinetic fate of paeoniflorin after intravenous administration. Method： Paeoniflorin and glycyrrhizin was administrated to rat via vena caudalis, and paeoniflorin in rat plasm was determined by RP HPLC method and internal standard method. All data were subsequently processed by the pharmacokinetic Software WinNonLin. The non-compartmental pharmacokinetic parameters of area under the plasma concentration-time curve （AUC/min·g· L^-1 ）, clearance （CL/mL·min·kg^-1 ） and volume of distribution （Vd/ mL·kg^-1） were calculated based on moment methods. Result： The values of AUC, Vd and CL was166. 81 ±26. 94, 394. 33 ± 29.52, 18.40 ±3. 12 in control group, respectively; however, the values of AUC, Vd, CL was 235.44 ±46. 48,266. 63 ±48.43 and 13. 16 ±2. 59 in experimental group. Conclusion： Glycyrrhizin significantly influenced the pharmacokinetic fate of paeoniflorin, increasing the value of AUC and decreasing CL and Vd.

关 键 词：芍药苷 甘草酸 HPLC 药代动力学 

分 类 号：R285.5[医药卫生—中药学]