LC/MS^n鉴定咖啡酸在大鼠体内的代谢产物  

The Characterization of the Metabolites of Caffeic Acid in Rats by LC/MS^n

作　　者：苏美英[1] 周婷婷[1] 周茂金[1] 

机构地区：[1]山东省泰安市中心医院,山东泰安271000

出　　处：《中国现代应用药学》2009年第6期501-505,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的研究咖啡酸(CA)在大鼠体内的代谢产物。方法大鼠灌胃(50mg·kg-1)给予CA后采集0～4h尿样,用电喷雾离子阱多级质谱法对CA在大鼠体内的代谢产物进行分析。结果大鼠灌胃给予CA后,在体内可测到2个原形药的甲基化代谢物、2个原形药的单葡萄糖醛酸结合物、1个原形药的双葡萄糖醛酸结合物、2个原形药的单硫酸结合物、2个甲基化物的葡萄糖醛酸结合物和2个甲基化物的硫酸结合物。结论CA在大鼠体内广泛代谢,其代谢物的结构有待于进一步分析后确证。OBJECTIVE To investigate the metabolites of caffeic acid （CA） in rats. METHODS After an oral administration of CA （50 mg·kg^-1） to Wistar rats, 0-4 h rats urine was collected, and metabolites of CA in rats was determined by liquid chromatography tandem multi-stage mass spectrometry （LC/MS^n）. RESULTS The results revealed that two methylated derivatives （ferulic and isoferulic acid） of CA, two CA-glucuronides, two CA-sulfates, one CA-di-glucuronide, two methylated CA-glucuronides, two methylated CA-sulfates were found in rats urine. CONCLUSION CA was extensively metabolized in rats and structures of the metabolites will be further confirmed.

关 键 词：咖啡酸 电喷雾离子阱多级质谱法 代谢物 

分 类 号：R917.1[医药卫生—药物分析学]