高压氧对缺氧缺血新生大鼠脑组织存活素及细胞凋亡蛋白酶-3表达的影响  

Effect of hyperbaric oxygen on survivin and caspase-3 expression in neonatal rats with hypoxia-ischemia brain damage

作　　者：纪丽莉[1] 

机构地区：[1]莱州市人民医院,山东省莱州261400

出　　处：《中华航海医学与高气压医学杂志》2009年第3期161-163,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的观察高压氧（HBO）对新生大鼠缺氧缺血性脑损伤（hypoxia—ischemia brain damage，HIBD）后脑组织存活素（survivin）及细胞凋亡蛋白酶-3（caspase-3）表达的影响，并探讨其保护机制。方法144只7d龄SD大鼠随机分为对照组（n=16）、新生HIBD组（n=64）及HBO治疗组（n=64）。采用经典的Rice法制作缺氧缺血性脑损伤模型，应用Epics XL流式细胞仪检测细胞凋亡情况，并应用免疫组织化学法检测Survivin和Caspase-3表达变化。结果与对照组相比，HIBD组细胞凋亡率及Survivin、Caspase-3表达水平明显升高，组间比较差异有统计学意义（P〈0．05）。通过相关性分析，HBO治疗可促进存活素表达，其表达水平和caspase-3表达负相关。结论HBO可能通过增加抗凋亡因子Survivin表达来抑制Caspase-3激活，减少缺氧缺血性脑损伤后细胞凋亡，发挥神经保护作用。Objective To explore the effect of hyperbaric oxygen （HBO） on the expression of survivin and caspase-3 following hypoxia-ischemia brain damage （HIBD） in neonatal rats, and the mechanism involved in the protection of the neural system by HBO. Methods One hundred and forty-four （ aged seven days） rats were randomly divided into three groups, i.e. the control group （n = 16）, the HIBD group （n = 64） and the HBO group （n = 64）. An experimental HIBD model was developed by the Rice method. Following damage, samples were obtained for measuring apoptosis of cells with the Epics XL flow cytometer and survivin expression and easpase-3 proteins were measured with immuno-histochemistry. Results When compared with that of the control group, the number of apoptotie cells, survivin, and caspase-3 immunopositive cells all increased significantly in the HBID group, and statistical differences could be seen between groups （ P 〈 0.05 ）. Analyses showed that HBO could significantly enhance the expression of survivin, with negative correlation between the level of survivin expression and the activity of caspase-3. Conclusions Increased expression of survivin by HBO might be one of the mechanisms involved in the regulation of easpase-3 and inhibition of neural cell apoptosis in cortex, which might be protective to neurons.

关 键 词：高压氧 缺氧缺血性脑损伤 脑组织存活素 细胞凋亡蛋白酶-3 凋亡 

分 类 号：R686[医药卫生—骨科学]