蟾酥提取物长循环脂质体的制备及质量评价  

Preparation and evaluation of long-circulating liposomes of Venenum Bufonis extractive

作　　者：徐昕[1] 潘卫三[1] 聂淑芳[1] 奉建芳[2] 祝林[3] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]上海中医药大学,上海201203 [3]上海医药工业研究院,上海200437

出　　处：《沈阳药科大学学报》2009年第7期517-522,共6页Journal of Shenyang Pharmaceutical University

摘　　要：目的研究蟾酥提取物长循环脂质体(chan su long-c ircu lation liposom e,CSLLP)的制备方法,并对其质量进行评价。方法考察不同方法制备CSLLP的包封率,最终确定选用乙醇注入法制备CSLLP,正交设计优化处方,用两亲性聚乙二醇2000-二硬脂酰磷脂乙醇胺(mPEG2000-DSPE)修饰脂质体膜,透射电镜观察形态,激光散射法测定粒径大小及分布,采用葡聚糖凝胶柱层析法和HPLC法测定CSLLP的包封率和渗漏率。结果乙醇注入法制备的CSLLP包封率最高,所得脂质体外观圆整而均匀,渗漏率小,稳定性好,平均粒径为69.8 nm,药物平均包封率为95.54%。结论用乙醇注入法可制备包封率高、稳定性好的CSLLP。Objective To prepare and characterize the long-circulating liposomes of extractive of Venenum Bufonis. Methods Compare the entrapment efficiency of each method including thin-film dispersion method, diethyl ether injection method and ethanol injection method, the long-circulating liposomes were finally prepared by ethanol injection method. Amphipathic polyethylene glycol distearoyl phosphatidylethanolamine （mPEG2000-DSPE） was added to modify the membrane of the liposomes. The entrapment efficiency and percolation rate were determined by Sephadex G-50 column chromatographic analysis antigenic and HPLC method. Results The average diameter of the Venenum Bufonis liposomes was 69.8 nm and the entrapment efficiency of the liposomes was 95.54%. Conclusions The preparation of Venenum Bufonis liposomes was easy and practicable, pharmaceutical characterization shows that the Venenum Bufonis liposomes were stable.

关 键 词：蟾酥提取物 长循环脂质体 乙醇注入法 粒度分布 包封率 

分 类 号：R94[医药卫生—药剂学]