HPLC法同时测定十味活血丸中升麻苷和甘草苷的含量  

Determination of prim-O-glucosylcimifugin and liquiritin in Shiweihuoxue pill by HPLC

作　　者：巩克民[1] 邱颖[2] 金艺[2] 赵怀清[2] 赵祥敏[3] 

机构地区：[1]盐城卫生职业技术学院药学系,江苏盐城224000 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]沈阳药大药业有限责任公司,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2009年第7期551-554,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立同时测定十味活血丸中升麻苷和甘草苷含量的HPLC方法。方法采用HPLC法，色谱柱为Diamonsil C18（200mm×4．6mm，5m），流动相为乙腈-体积分数为0．5％的冰醋酸溶液（体积比为15：85），流速：1．0mL·min^-1，检测波长：276nm，柱温：40℃。结果十味活血丸中升麻苷和甘草苷与其他成分分离良好。升麻苷的质量浓度在2．86—28．6mg·L^-1（r=0．9997）、甘草苷的质量浓度在0．774—7．74mg·L^-1（r=0．9999）内与峰面积呈良好的线性关系．升麻苷和甘草苷的平均回收率分别为99．2％和99．0％，RSD（n=9）分别为1．8％和1．6％。结论该法可用于十味活血丸中升麻苷和甘草苷的含量测定。Objective To establish an HPLC method for simultaneous determination of prim-O-glucosylcimifugin and liquiritin in Shiweihuoxue （traditional Chinese medicine）pill. Methods The separation was performed on a Diamonsil C18（4. 6 mm × 200 mm,5 μm）column. The mobile phase was composed of acetoniWile and 0. 5 % （th） glacial acetic acid solution at the rate of 15：85. The column temperature was set at 40 ℃ and the flow rate was 1.0 mL·min-1i, with UV detection at 276 nm. Results The numbers of theoretical plates calculated for prim-O-glucosylcimifugin and liquiritin were 4 600 and 5 500 ,respectively. The regression equation of prim-O-glucosylcimifugin was A = 8. 910 × 10^3 p ＋ 1. 812 ×10 （ r = 0. 999 7 ）, which was linear over the ranger of 2. 86-28.60 mg·L^-1. The regression equation of liquiritin was A = 2. 548 ×10^4 p ＋8.741 × 10 （ r = 0. 999 9）, which was linear over the ranger of 0. 774 - 7.74 mg·L^-1. The average recovery was 99. 2% （RSD = 1.8% ）for prim-O-glucosylcimifugin ,99.0% （RSD = 1.6% ）for liquiritin. Conclusions The method is simple and accurate, and can be used as the method for quality assessment of Shiweihuoxue pill.

关 键 词：十味活血丸 高效液相色谱法 升麻苷 甘草苷 

分 类 号：R917[医药卫生—药物分析学]