Ang-Ⅱ对血管内皮细胞线粒体膜电位的影响及阿托伐他汀的保护作用  

Effects of angiotensin-Ⅱ on MMP in VEC and protection of atorvastatin

作　　者：韩磊[1] 李鸣皋[1] 叶平[2] 

机构地区：[1]海军总医院航空潜水医学中心,北京100037 [2]中国人民解放军总医院老年心血管2科

出　　处：《临床心血管病杂志》2009年第7期544-546,共3页Journal of Clinical Cardiology

基　　金：海军后勤科研基金(No:00-3321)

摘　　要：目的:观察血管紧张素-Ⅱ(Ang-Ⅱ)对血管内皮细胞线粒体膜电位的影响及阿托伐他汀的保护作用。方法:将血管内皮细胞分为:空白对照组(仅给予细胞培养液)、Ang-Ⅱ组(细胞培养液中加入Ang-Ⅱ,使其终浓度为10-7mol/L)、Ang-Ⅱ加小剂量阿托伐他汀组(在单纯Ang-Ⅱ组的基础上加入阿托伐他汀,使阿托伐他汀的终浓度为0.1μmol/L)、Ang-Ⅱ加大剂量阿托伐他汀组(在单纯Ang-Ⅱ组的基础上加入阿托伐他汀,使阿托伐他汀的终浓度为1μmol/L)。用激光共聚焦显微镜测量各组细胞的线粒体膜电位水平。结果:①Ang-Ⅱ组的线粒体膜电位显著低于空白对照组(P<0.01);②Ang-Ⅱ加阿托伐他汀组的线粒体膜电位显著高于Ang-Ⅱ组(P<0.01)。③Ang-Ⅱ加大剂量阿托伐他汀组的线粒体膜电位水平高于Ang-Ⅱ加小剂量阿托伐他汀组(P<0.05)。结论:Ang-Ⅱ可引起血管内皮细胞线粒体膜电位的显著降低,而阿托伐他汀可呈剂量依赖性的逆转Ang-Ⅱ的这一作用。Objective：To investigate the effects of angiotensin- Ⅱ（Ang Ⅱ ） on mitochondria membrane potential in vascular endothelial cell （VEC） and protection of atorvastatin. Methods： Cultured VEC were divided into 4 groups：control group （only given cell culture medium） ; Ang- Ⅱ group （Ang- v was given with the last concentration be 10^-7 mol/L） ; Ang- Ⅱ ＋ low dosage atorvastatin group （atorvastatin were given with the last concentration be 0.1 μmol/L at the base of Ang-Ⅱ group; AngⅡ ＋ high dosage atorvastatin group （atorvastatin were given with the last concentration be 1 μmol/L at the base of Ang-Ⅱ group）, mitochondria membrane potential were measured by laser scanning confocal microscopy. Results：（1）mitoehondria membrane potential of Ang Ⅱ group significantly lower than that of control group（P〈0.01）; （2）mitochondria membrane potential of Ang-Ⅱ ＋atorvastatin group significantly higher than that of Ang-Ⅱ group （P〈0.01）; （3）mitochondria membrane potential of Ang- Ⅱ ＋atorvastatin group higher than that of Ang-Ⅱ group （P〈0.05）. Conclusion：Ang-Ⅱ induce obvious changes of mitochondria membrane potential in VEC and atorvastatin inhibits it in a dose-dependent manner.

关 键 词：血管紧张素-Ⅱ 血管内皮细胞 膜电位 线粒体 阿托伐他汀 

分 类 号：R347[医药卫生—基础医学]