新法制备动脉脱细胞基质的实验研究  

A new method of making arterious acellular scaffold

作　　者：马良龙[1] 黄惠民[2] 李建华[1] 朱雄凯[1] 张泽伟[1] 

机构地区：[1]浙江大学医学院附属儿童医院浙江省医学重点学科心胸外科,杭州310003 [2]上海儿童医学中心心胸外科

出　　处：《中华小儿外科杂志》2009年第7期468-471,共4页Chinese Journal of Pediatric Surgery

基　　金：基金项目：浙江省自然科学基金（No.Y2080144）、浙江大学医学院科研启动基金（No.491040-542921）

摘　　要：目的建立一种新的动脉血管脱细胞基质制备方法。方法取犬动脉，应用自行设计的胰酶、SDS和反复冻融的多步骤法制备犬动脉脱细胞基质。电镜、病理及力学检测脱细胞效果及力学特性。将猪内皮祖细胞种植于脱细胞基质，构建组织工程血管并移植到骨髓供体猪颈动脉或肺动脉。结果应用胰酶消化、SDS漂洗及反复冻融处理后，保持了犬动脉的管形，细胞成分完全去除，最大断裂负荷、拉伸长度与自体动脉类似。种植内皮祖细胞后形成完整的内皮细胞层。移植到颈动脉后出现狭窄、堵塞。移植到肺动脉后血管通畅。结论胰酶、SDS和反复冻融结合制备血管脱细胞基质，能够完全去除细胞成分，保留血管支架结构。Objective To present a new method of making arterious acellular scaffold. Methods Trypsin and SDS were used to treat canine artery at first, and then the arteries were frozen and thawed. The biological and biodynamic characters of acellular scaffold were identified. Porcine endothelial progenitor cells were seeded on the acellular scaffold. Then the engineered vessels were transplanted into the pulmonary artery or the carotid artery of the Cell donator pig. After 3 and half months, the engineered vessels were explanted and the biological and mechanic characters of these vessels were analyzed again. Results Trypsin digestion and SDS cleaning removed all the cell component of canine artery. The porosity was reserved. Endothelial progenitor cells proliferated well on the heterogeneous acellular scaffold and formed a mono-layer on the surface of scaffold. After in position transplantation, the engineered vessel in pulmonary functioned well. Mono-layer of endothelial cell could be seen on the internal surface. Conclusions Trypsin digestion, SDS cleaning and freezing thawing can remove all cellular component of artery, which can be used as an excellent acellular vessel scaffold.

关 键 词：内皮细胞 组织工程 血管 制备方法 力学特性 

分 类 号：R686[医药卫生—骨科学]