利用EST-SSR分子标记研究鲤的饲料转化率性状  

Studies on feed conversion ratio trait of common carp(Cyprinus carpio L.) using EST-SSR marker

作　　者：李鸥[1,2] 曹顶臣[1] 张研[1,3] 顾颖[1,2] 张晓峰[1] 鲁翠云[1] 孙效文[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所及农业部北方鱼类生物工程育种重点开放实验室,黑龙江哈尔滨150070 [2]大连水产学院生命科学与技术学院,辽宁大连116023 [3]中国水产科学研究院水产应用基因组研究中心,北京100144

出　　处：《水产学报》2009年第4期624-631,共8页Journal of Fisheries of China

基　　金：国家重点基础研究发展计划(2004CB117405)

摘　　要：饲料转化率是重要的经济性状,通过QTL定位获得的紧密连锁标记以及相关基因,是遗传育种的重要工具。本文利用70个鲤的EST-SSR,对鲤全同胞家系92个体的基因组DNA进行扫描,得到符合1∶1孟德尔分离类型的位点48个,3∶1孟德尔分离类型的位点22个。采用拟回交策略对符合1∶1分离的标记与鲤饲料转化率性状进行单标记回归分析。结果表明:HLJE335、HLJE253、HLE547、HLJE92、HLJE203、HLJE231等6个标记与饲料转化率性状相关(P<0.05)。其中,HLJE547和HLJE203两个标记与饲料转化率相关性达极显著水平(P<0.001),分别解释了表型变异的11.00%、11.00%。应用NCB I数据库,分别对与性状极显著相关的2个EST-SSR标记进行同源性比对,发现标记HLJE547与斑马鱼基因组中编码ATPase,H+transporting,lysosomal的核酸序列同源(同源性为74%),与斑马鱼编码的ATPase,H+transporting,lysosomal的蛋白序列同源(同源性为92%)。推测ATPase可能通过影响鲤消化与吸收等生物学机制,从而影响鲤的饲料转化效率。Feed conversion ratio is very important economic traits. The improvement of it can not only save the feed, reduce the cost, but can also improve the ecomonic efficiency. There were a great deal of studies relating to feed conversion ratio of QTL in the field of farming. But few were reported on fish, especially on common carp. The linked markers and genes from the analysis of QTL are very important tools for fish breeding. Seventy EST-SSR markers were selected to analyze the genomic DNA of ninety-two progenies derived from the common carp （ Cyprinus carpio L. ） full-sib family, which come from the cross between progenies of Barbless carp and Hebao-cold tolerance red carp. 70 makers segregate according to Mendel＇s Law, of which 48 segregate in 1 ： 1 model and other 22 in 3： 1 model. Ma kers of 1 ： 1 segregation model were used to made marker regression analysis based on pseudo-testcross strategy using Windows Map Manager 2.0. Six loci were discovered that had impact on feed conversion efficiency （ HLJE335, HLJE25, HLE547, HLJE92, HLJE203, HLJE231 ） and two of them had a significant impact on this trait. EST-SSR markers were used to screen the nucleotide and protein database in the National Center for Biotechnology Information （NCBI） in order to find the homologies. High sequence homologies of HLJE547 marker were observed with the nucleotide sequence of ATPase, H ＋ transporting, lysosomal gene of zebra fish and protein sequence of zebra fish ATPase, H＋ transporting, lysosomal protein. Homologous were 74%, 94% respectively. ATPase, H＋ transporting, lysosomal protein is a component of vacuolar ATPase （V- ATPase）, which were detected in iontransporting epithelium of the a blowfly rectum. In addition, there were a great deal of lysosome in epithelium of the Tachypleus tridentatus Leach rectum. Fish as the first key link of craniota may have some similar function with invertebrate. This indicates that V-ATPase may affect the function of digestive system through affecting the H ＋ transpo

关 键 词：鲤 EST—SSR 饲料转化率 标记回归分析 

分 类 号：S963[农业科学—水产养殖]