不同水温下强力霉素在斑点叉尾鮰体内的残留消除规律  

Studies on elimination regularity of doxycycline residues in Ictalurus punctatus at different temperatures

作　　者：丁俊仁[1] 汪开毓[1] 艾晓辉[2] 刘永涛[2] 袁科平[2] 袁丹宁[3] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]中国水产科学研究院长江水产研究所农业部淡水鱼类种质监督检验测试中心,湖北荆州434000 [3]华中农业大学水产学院,湖北武汉430070

出　　处：《水产学报》2009年第4期672-678,共7页Journal of Fisheries of China

基　　金：国家“十一五”科技支撑计划重大项目(2006BAK02A22);中国水产科学研究院专项资金项目(2007B003)

摘　　要：研究不同水温(18±1)℃和(28±1)℃下,强力霉素在斑点叉尾鮰体内的残留消除规律。以20 mg/kg鱼体重连续口灌斑点叉尾鮰5 d,于停药后第1、3、5、7、9、12、15、24、30、40天分别将斑点叉尾鮰处死后取肌肉(加皮)、肝脏、肾脏3种组织,采用高效液相色谱紫外检测法测定斑点叉尾鮰组织中强力霉素。结果表明,强力霉素在斑点叉尾鮰体内的消除速度与水温有密切关系,不同水温下相同组织,相同水温下不同组织中强力霉素的消除速率不同(P<0.05)。高水温时强力霉素在斑点叉尾鮰体内消除快,表明水温对斑点叉尾鮰体内的药物代谢有明显的影响,强力霉素残留的消除速度随水温降低而减慢;与其他组织相比,强力霉素在肝脏中的消除最慢。因此,若将肝脏作为强力霉素在斑点叉尾鮰体内残留的靶组织计算休药期,在(18±1)℃和(28±1)℃时,按欧盟和中国规定的动物组织中强力霉素在肝脏中最高残留限量300μg/kg计算,从食品安全角度来分析,建议休药期分别为55 d和30 d。若按强力霉素在可食组织肌肉(加皮)中最高残留限量300μg/kg计算休药期,建议休药期分别为22d和19 d。本研究旨为不同水温条件下制定强力霉素在斑点叉尾鮰体内的残留限量和休药期提供理论依据。Doxycycline （DOTC） is a tetracycline broad-spectrum antibiotic widely used for the treatment of systemic bacterial infections. The broad-spectrum antibiotic doxycycline （DOTC） has commonly been used in channel catfish aquaculture to prevent and treat bacterial diseases. In order to study the tissue distribution and elimination of doxycycline （DOTC） in channel catfish （ Ictalurus punctatus ）, continuous （ for 5 days） oral administration（20 mg/kg body weight） of DOTC has been investigated in healthy channel catfish at （ 18 ± 1 ） ℃ and （ 28 ± 1 ） ℃ water temperature. This is first report on tissue distribution and residues of DOTC in channel catfish. After repeated oral administration for 5 consecutive days, channel catfish （Ictalurus punctatus） were killed and the muscle and skin, liver, kidney tissues were collected after 1,3,5, 7,9,12,15,24,30,40 d of oral administration. Doxycycline （DOTC） in tissues was determined by using high performance liquid chromatography （HPLC） with UV detection. The mobile phase was 0.01 mol/L oxalic acid： acetonitrile： methanol （70： 20： 10, V/V/V） and UV detection set at 350 nm. Analyte was extracted with Mcllvaine buffer-EDTA extraction, whose pH was 4.0. DOTC was eluted from Oasis HLB column with 2 mL methanol. The standard curves were linear in the investigated ranges, 0.05 - 10.0 p.g/g of DOTC in tissues. The correlation coefficient for DOTC was both r≥0. 9964. The recovery of DOTC was from 75.13% to 92.05%. The within-day and day-to-day precision expressed by CV was less than 10% at two drug levels （0.1 and 1.0 μg/g）. The detection limit for DOTC in muscle and skin, liver, kidney was 10,20 and 20 μg/kg respectively. The results show that the depletion speed of DOTC in channel catfish closely relates with the water temperature. The elimination rate was markedly different at different water temperature in the same tissues. The depletion of DOTC residue in channel catfish was faster at high water temperature. To

关 键 词：斑点叉尾鲴 强力霉素 残留 消除规律 

分 类 号：S948[农业科学—水产养殖] R969.1[农业科学—水产科学]